Phage display-derived peptides

Ladner RC, Sato AK, Gorzelany J, et al. Phage display-derived peptides as therapeutic alternatives to antibodies. Drug Discov Today 2004 9 525-9. 

Molenaar, T J M, Appeldoom, C C M, de Haas, SAM, Michon, I N M, Bonnefoy, A, Hoylaerts, M F, Pannekoek, H, van Berkel, T J C, Kuiper, J, Biessen, E A L, Specific inhibition of P-selectin-mediated cell adhesion by phage display-derived peptide antagonists. Blood, 100, 3570-3577, 2002. 

J.M. Davies, et al., (1999). Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopes. Mol. Immunol. 36, 659-667. 

Table 4. Selected examples of short peptide motifs derived from phage display peptide libraries (adapted from [116]) ...

Table 4 lists some examples of proteins that bind short peptide motifs derived from phage display peptide libraries [66,116-121]. Of particular interest is the use of phage display peptide libraries for screening peptides that bind to integrins. Each of the integrins and has a slightly different... 

Fig. 5.12. Strategy for the selection of phage-displayed staphylococcal nuclease (SNase) with a DNA substrate derivative featuring a biotin (Bt) on one end and a basic peptide on the other [65], The substrate is attached to the phage via an acidic peptide and the phage is captured with immobilised streptavidin (Sv).

Gttii bound to GEF peptides derived from phage-display (KB-752) and the dopamine D2-receptor (D2N) roles of fS3/a2 loop and fS6 strand in Ga GDP release 2HLB... 

Venkztesh, N., Zaltsman, Y., Somjen, D., Gayer, B., Boopathi, E., Kasher, R., Kulik, T., Katchalski-Katzir, E., and Kohen, E, 2002. A synthetic peptide with estrogen-like activity derived from phage-display peptide library. Peptides, 23, pp. 573-580. 

Chang, W. J., Kishikawa-Kiyama, M., Shibata, Y., Lee S. Y., Abiko, Y. (2004). Inhibition of Porphyromonas gingivalis hemagglutinating activity by synthetic peptides derived from phage display selection using MAb against the recombinant outer membrane protein. Hybrid Hybridom., 23, 352-356. 

The structure of p53 in complex with hdm2 [40] and the initial data obtained with p53-derived peptides [36, 39] indicate that peptides can be used to study this interaction to establish a pharmacophore model. Phage display experiments [47] allowed the identification of a 12-mer phage-derived peptide (peptide 2, Table 15.5-2) that is 29 times more potent than the wild-type peptide (peptide 1, Table 15.5-2) [48], Peptide 2 was truncated to eight residues, leading to a peptide with micromolar activity (peptide 3, Table 15.5-2) [48]. It should... 

Kelly etalS presented a complete process validation smdy for a peptide ligand that was derived from a phage-displayed peptide library for the purification of recombinant B-Domain Deleted factor vm (BDDrFVin). The peptide column was reused 26 times without any loss in resin performance. The lifetime study was not extended further because there is no requirement for the process economics to go beyond this lifetime. 

MIPD has also been utilized for potential diagnostic use in Alzheimer s disease. Willbold et al. used the all-o version of Ap(l-42) amyloid peptide as a target to screen a commercial phage display library to generate a dominant consensus sequence whose all D-amino acid analog o-pep bound the native AP(l-42) sequence with submicromolar affinity [181]. In addition, a fluorescein-labeled derivative of o-pep was shown to stain native Ap amyloid fibrils specifically, suggesting it may be useful as a diagnostic probe for amyloid plaques. 

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