Disc diffusion method

Detecting evolutionary hot spots of antibiotic resistance in Europe Disc diffusion method Genes encoding dihydrofolate reductases confers resistance to trimethoprim... 

In sensitivity tests based on disc diffusion methods, the clear zone of inhibition around each individual disc was measured in three different directions to obtain the mean value of each set. 

Resistant by Kirby-Bauer disc diffusion method to penicillin G (P), Tetracycline (T), Clindamycin (C), Novobiocin (N), Kanamycin (K), Chloroamphenicol (Ch), Erythromycin (E). 

An antibacterial assay using the disc-diffusion method found that the stems and the roots of H. capitellata showed weak to moderate activities against the tested bacteria, Bacillus subtilis B28, Bacillus subtilis B29, Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) while the leaves showed weak activity toward Bacillus subtilis B28, MRSA and P. aeruginosa only. However, the roots of H. dichotoma showed moderate antibacterial properties against all four bacteria. All other extracts did not exhibit any antibacterial properties. Table 10 shows the inhibition zones of the methanolic extracts of some Hedyotis species as measured by the disc-diffusion method. 

Table 10 Inhibition zones (mm) of methanolic extracts of Hedyotis species as measured by the disc-diffusion method.

Antibacterial efficacy decreased after non-ionic and anionic lanndering of the fabric. In particular, the antibacterial activity of Ppy(Cl) samples was severely degraded by anionic laundering. The antibacterial activity of Ppy has been investigated against Streptococcus pneumoniae (RCMB 010010), Enterococcus faecalis (RCMB 010068) and Staphylococcus aureus (RCMB 010028), i.e.. Gram-positive bacteria. The agar disc diffusion method was used to determine the antibacterial activity and ampicillin... 

The improved antibacterial activity of the BC-chitosan composite via the impregnation approach was reported. Firstly, the antimicrobial evaluation was performed by the direct contact of the composite with coli and S. aureus. The growth reductions of 99.9% were observed in both cultures. Secondly, the evaluation was performed by the disc diffusion method. Because of a tight incorporation between chitosan and BC, it resulted in no chitosan diffusion out of the composite. Therefore, no inhibitory clear zone was found [20],... 

The antibacterial activity of the compounds is determined by the disc diffusion method [42]. The bacteria are cultured in nutrient agar medium and used as inoculum for the study. Bacterial cells are swabbed onto nutrient agar medium (prepared from NaCl (5.0 g), peptone (5g), beef extract powder (3g), yeast extract powder (3g), and agar (20 g) in 1000 ml distilled H2O) pH 7.5 0.2 in Petri dishes. The test solutions are prepared in distilled water to a final concentration of 1 %, 2%, and 4% and then appHed to filter paper discs (Whatman No. 4, 5 mm diameter). These discs are placed on the already seeded plates and incubated at 35 2 °C for 24 h. The zones of inhibition around the discs are measured after 24 h. Co-trimoxazole is used as a standard positive control (Table 6.9). 

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