Antibacterial activity disc diffusion agar method

Antibacterial efficacy decreased after non-ionic and anionic lanndering of the fabric. In particular, the antibacterial activity of Ppy(Cl) samples was severely degraded by anionic laundering. The antibacterial activity of Ppy has been investigated against Streptococcus pneumoniae (RCMB 010010), Enterococcus faecalis (RCMB 010068) and Staphylococcus aureus (RCMB 010028), i.e.. Gram-positive bacteria. The agar disc diffusion method was used to determine the antibacterial activity and ampicillin... 

The antibacterial activity of the compounds is determined by the disc diffusion method [42]. The bacteria are cultured in nutrient agar medium and used as inoculum for the study. Bacterial cells are swabbed onto nutrient agar medium (prepared from NaCl (5.0 g), peptone (5g), beef extract powder (3g), yeast extract powder (3g), and agar (20 g) in 1000 ml distilled H2O) pH 7.5 0.2 in Petri dishes. The test solutions are prepared in distilled water to a final concentration of 1 %, 2%, and 4% and then appHed to filter paper discs (Whatman No. 4, 5 mm diameter). These discs are placed on the already seeded plates and incubated at 35 2 °C for 24 h. The zones of inhibition around the discs are measured after 24 h. Co-trimoxazole is used as a standard positive control (Table 6.9). 

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