Total digestion

Figure 3. Chromatogram of the EPG-digested total alkali extract on an HW-55 S gel filtration column.

Mineral samples such as rock, soil, and sediments require more aggressive digestion. Total sample dissolution may be done by several methods, and... 

Water and wastes Acid digestion filtration (dissolved) acid digestion (total recoverable) ICP-MS (total uranium) 0.1 pg/L 105-110% Long and Martin 1991 (EPA Method 200.8)... 

For the isolation of psliG2, Hindlll digested total DNA was separated on a 10 -30 % sucrose gradient. Fractions enriched in fragment sizes of 5 - 6 kb were subcloned in Bluescript KS. About 700 colonies were screened by Southern blot analysis with a psbG specific probe under low stingency conditions and six positive clones were identified. 

Southern blot analysis of undigested (0) and restriction enzyme digested total Synechocystis DNA (1 Hindlll, 2 EcoRI,... 

Soybean-Casein Digest Total count Bacteria <3 days at 30-35 °C... 

Utihty production of biomass-fueled electric power is much less than nonutiUty production. In early 1985, there were only 18 faciUties having a total capacity of 245 MW, ie, nine fueled with wood (180.7 MW), five fueled with MSW (33.8 MW), two fueled with agricultural residues (22.5 MW), and two fueled with digester gas (8 MW) (112,113). The largest was the 50-MW plant in Burlington, Vermont (114). 

The total phosphoms content of the sample is determined by method AOCS Ja 5-55. Analysis of phosphoUpid in lecithin concentrates (AOCS Ja 7-86) is performed by fractionation with two-dimensional thin-layer chromatography (tic) followed by acid digestion and reaction with molybdate to measure total phosphorous for each fraction at 310 nm. It is a semiquantitative method for PC, PE, PI, PA, LPC, and LPE. Method AOCS Ja 7b-91 is for the direct deterrnination of single phosphoHpids PE, PA, PI, PC in lecithin by high performance Hquid chromatography (hplc). The method is appHcable to oil-containing lecithins, deoiled lecithins, lecithin fractions, but not appHcable to lyso-PC and lyso-PE. 

Arsenic. Total arsenic concentration can be determined by reduction of all forms to arsine (AsH ) and collection of the arsine in a pyridine solution of silver diethyldithiocarbamate. Organoarsenides must be digested in acidic potassium persulfate prior to reduction. The complex that forms is deep red, and this color can be measured spectrophotometricaHy. Reduction is carried out in an acidic solution of KI—SnCl2, and AsH is generated by addition of 2inc. 

Cobalt is one of twenty-seven known elements essential to humans (28) (see Mineral NUTRIENTS). It is an integral part of the cyanocobalamin [68-19-9] molecule, ie, vitamin B 2> only documented biochemically active cobalt component in humans (29,30) (see Vitamins, VITAMIN Vitamin B 2 is not synthesized by animals or higher plants, rather the primary source is bacterial flora in the digestive system of sheep and cattle (8). Except for humans, nonmminants do not appear to requite cobalt. Humans have between 2 and 5 mg of vitamin B22, and deficiency results in the development of pernicious anemia. The wasting disease in sheep and cattle is known as bush sickness in New Zealand, salt sickness in Florida, pine sickness in Scotland, and coast disease in AustraUa. These are essentially the same symptomatically, and are caused by cobalt deficiency. Symptoms include initial lack of appetite followed by scaliness of skin, lack of coordination, loss of flesh, pale mucous membranes, and retarded growth. The total laboratory synthesis of vitamin B 2 was completed in 65—70 steps over a period of eleven years (31). The complex stmcture was reported by Dorothy Crowfoot-Hodgkin in 1961 (32) for which she was awarded a Nobel prize in 1964. 

Concentration of WTE Incinerators The total number of municipal waste incinerator facihties as hsted in the Solid Waste Digest, vol. 4, no. 9 September 1994 (a publication of Chartwell Information Publishers of Alexandria, VA) is 62. See Table 25-69, which covers over 200 existing units. The wastes burned in these facilities totals 8.44 percent of total municipal wastes managed in landfills, incinerators, and transfer stations. This amounts to 88,470 tons per day combusted municipal waste. 

Elemental analysis of organie and inorganie samples requires partial or total dissolution of the sample prior to speetrometrie analysis. Mierowave-assisted extraetion (MAE) provides an alternative to traditional digestion methods and it is reeognized as one of the most effieient methods for the dissolution of soil samples. 

Sharp decreases in total gas production may indicate toxicity in the digester. The gas is usually about 70 percent methane, about 30 percent carbon dioxide and inert gases such as nitrogen. An increasing percentage of carbon dioxide may be an indication that the digestion process is not proceeding properly. 

A 0.2-g sample of amylopectin was analyzed to determine the fraction of the total glucose residues that are branch points in the structure. The sample was exhaustively methylated and then digested, yielding 50 /tmol of 2,3-dimethylglucose and 0.4 /tmol of 1, 2, 3, 6-tetramethylglucose. 

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