Diagnostic pathology

DeLellis RA, Sternberger LA, Mann RB, et al. Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute. Am. J. Clin. Pathol. 1979 71 483-488. 

Chaurand P, Sanders ME, Jensen RA, et al. Proteomics in diagnostic pathology, profiling and imaging proteins directly in tissue sections. Am. J. Pathol. 2004 165 1057-1068. 

In view of disadvantages associated with the use of cryosections in immunohistochemistry, the general trend is that most immunohistochemical investigations both in diagnostic pathology and in basic research studies are carried out on formaldehyde-fixed paraffin-embedded tissue sections. 

Coon, J. S., Landay, A. L., and Weinstein, R. S. (1986) Flow cytometric analysis of paraffin-embedded tumors implications for diagnostic pathology. Human Pathol. 17, 435 37. 

Presently, nonradioactive probes, especially biotin or digoxigenin, are favored because they are less hazardous to work with, can be more rapidly developed, and provide better spatial resolution. Thus, introduction of nonradioactive detection systems has made ISH, using formalin-fixed and paraffin-embedded tissues, more accessible for application to molecular cell biology and diagnostic pathology. However, radioactive detection systems are more sensitive than nonradioactive probes, especially oligonucleotide probes used instead of cRNA probes (Sperry et al., 1996). 

Hayat, M. A. 2000b. Care and use of microwave oven for diagnostic pathology. Microsc. Today April issue No. 00-3 29. 

Some practical applications of immunofluorescence in diagnostic pathology are ... 

Jennette JC and Wick MR. Immunohischemical techniques. In Immunohistology in diagnostic pathology , Ed. Jennette JC. CRC Press. Boca Raton, FL. USA. 1988. Pp. 1-29. 

Rdcken, C., Meier, H., Klanck, S., Wolff, S., Malfertheiner, P., Roess-ner. A. Large-needle biopsy versus thin-needle biopsy in diagnostic pathology of liver diseases. Liver 2001 21 391 —397... 

Mandell, J. W., Phosphorylation state-specific antibodies applications in investigative and diagnostic pathology, Am. J. Pathol. 2003, 163, 1687-1698... 

A simple heat-induced AR technique is now widely applied in pathology. 40,57,59 Successful application of the AR technique for routine IHC staining of formalin-fixed tissues in diagnostic pathology has rendered the search for alternative fixatives to replace formalin less... 

In summary, IHC, ISH, and PCR should be regarded as complementary diagnostic methods for use in the diagnostic pathology laboratory. The laboratory must... 

Wick MR. Antibodies to desmin in diagnostic pathology. In Wick MR, Siegal GP, eds. Monoclonal antibodies in diagnostic immunohistochemistry. New York Marcel Dekker 1988 93-114. 

Kahn HJ, Marks A, Thom H, et al. Role of antibody to SlOO protein in diagnostic pathology. Am J Clin Pathol. 1983 79 341-347. 

Kaiserling E. Immunohistochemical identification of lymph vessels with D2-40 in diagnostic pathology. Pathologe. 2004 25 362-374. 

Bostwick DG. Prostate-specific antigen. Cuttent role in diagnostic pathology of prostate cancet. Am J Clin Pathol. 1994 102 S31-S37. 

The vesicle-associated membrane proteins (VAMP or synaptobrevin) occur in three isoforms and are proteins that are anchored to the cytoplasmic portion of synaptic membrane vesicles and secretory granules. VAMP2 and 3 are present in pancreatic beta cells, but the roles of this family of proteins have not been widely studied as markers of NE tumors. In contrast to synaptophysin and other synaptic vesicle proteins, SNAP-25 (synaptosomal protein of 25 KD) and syntaxin are present in the plasma membranes. At present, there are few studies on the application of these markers in diagnostic pathology... 

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