Detection strategies

Many nucleic acid detection strategies use target amplification, signal amplification or both. Invader, branched DNA (bDNA) and rolling circle amplification (RCA) are three approaches. 

Detection strategy Highly flexible (sequential detection) On-line... 

Sadaghiani AM, Verhelst SHL, Bogyo M (2007) Tagging and detection strategies for activity-based proteomics. Curr Opin Chem Biol 11 20-28... 

Silica particles have been exploited in virtually every assay or detection strategy that polymer particles have been used in for bioapplication purposes. Recently, fluorescent dye-doped silica nanoparticles have been developed by a number of groups that have similar fluorescence characteristics to quantum dot nanocrystals (Chapter 9, Section 10). Fluorescent silica nanoparticles can be synthesized less expensively than quantum dots due to the fact that the silica particles incorporate standard organic dyes (Ow et al., 2005 Wang et al., 2006) and are not dependent on making reproducible populations of semiconductor particles with precise diameters to tune emission wavelengths. 

A second variant detection strategy involves directed resequencing of selected genomic regions from different individuals, usually between 50 and 100 unrelated individuals. Many of these efforts have screened a subset of the DNA Polymorphism Discovery Resource collection, a set of 450 samples selected by the NIH to be a sample of the ethnic diversity of the U.S. popu-... 

To be able to effectively respond to cyber attacks, establish an intrusion detection strategy that includes alerting network administrators of malicious network activity originating from internal or external sources. Intrusion detection system monitoring is essential twenty-four hours a day this capability can be easily set up through a pager. Additionally, incident response procedures must be in place to allow an... 

The unique practical properties of adsorption have promoted its extensive use in genetic analysis. The disadvantages of adsorption with respect to covalent immobihzation are mainly that (1) nucleic acids may be readily desorbed from the substrate, and (2) base moieties may be unavailable for hybridization if they are bonded to the substrate in multiple sites [34]. However, the electrochemical detection strategy based on the intrinsic oxidation of DNA requires the DNA to be adsorbed in close contact with the electrochemical substrate by multi-point attachment. This multi-site attachment of DNA can be thus detrimental for its hybridization but is crucial for the detection based on its oxidation signals. 

Bober, M. A., Kurth, M. J., Milco, L. A., Roseman, D. M., Miller, R. B. and Segal, H. J. 1991. A pyrrolizidine alkaloid-enzyme-linked immunosorbent-assay detection strategy. ACS Symposium Series, 451 176-183 and, Bober, M. A., Milco, L. A., Miller, R. B., Mount, M., Wicks, B. and Kurth, M. J. 1989. A competitive enzyme-linked immunosorbent-assay (ELISA) to detect retronecine and monocrotaline in vitro. Toxicon, 27(9) 1059-1064. 

Lane S, Boughtflower B, Mutton I et al. Toward single-calibrant quantification in HPLC. A comparison of three detection strategies Evaporative light scattering, chemiluminescent nitrogen, and proton NMR. Analytical Chemistry 77, 4354 365, 2005. 

From the consumer s point of view as well, controls on food products are needed, from a food safety and traceability perspective. The problem of adulteration and falsification of food has ancient roots which date back to the Roman Age and continue, through the Middle and Modem Ages, till nowadays the deployment of new analytical tools for fraud detection has caused a parallel progress in the adulteration procedures, which has gradually evolved from coarse and rudimentary systems to highly sophisticated and scarcely detectable strategies. 

Fig. 2. The principles of protein microarray detection strategies, (a) Label-based detection method, (b) label-free based detection methods.

Warren, K. C., Coyne, K. J., Waite, J. H., and Cary, S. C. 1998. Use of methacrylate deembedding protocols for in situ hybridization on semithin plastic sections with multiple detection strategies. J. Histochem. Cytochem. 46 149-155. 

The sensitivity of the detection is usually improved by the silver enhancement method. A better detection limit was reported when a silver enhancement method was employed, based on the precipitation of silver on AuNPs tags and its dissolution (in HNO3) and subsequent electrochemical potentiometric stripping detection [43]. The new silver-enhanced colloidal gold stripping detection strategy represented an attractive alternative to indirect optical affinity assays of nucleic acids and other biomolecules. 

The previous section clearly demonstrated the need for CD measurements on a time-resolved basis. Both stopped-flow and chromatographic applications require that CD measurements be obtained on the millisecond, or longer time scale. For this time domain, modification of conventional CD systems is appropriate and this approach has met with excellent success. However, there are a number of important applications where CD information is required in the microsecond to picosecond time domain. Certainly this time regime can not be accessed by simple modification of conventional CD detection strategies and new approaches have therefore been devised to extend CD measurements into the microsecond, and below, time domain. The crux of this section will be to consider CD detection strategies which are designed to improve the measurement SNR, and therefore allow extension into new domains such as time-resolved CD studies. 

Sharing the incident details with hundreds of mechanics, chemical process operators, and supervisors within the chemical complex. This training included stressing the proper flammable gas detection strategy. [3]... 

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