Proteomics activity-based

Sadaghiani AM, Verhelst SHL, Bogyo M (2007) Tagging and detection strategies for activity-based proteomics. Curr Opin Chem Biol 11 20-28... 

Speers, A.E., and Cravatt, B.F. (2004a) Chemical strategies for activity-based proteomics. Chem. Bio. Chem. 5, 41 17. 

Yang P-Y, Liu K, Ngai MH, Lear MJ, Wenk MR, Yao SY (2010) Activity-based proteome profiling of potential cellular targets of orlistat - an FDA approved drug with anti-tumor activities. J Am Chem Soc 132 656-666... 

Orru, S., Pagnozzi, D., and Pucci, R, Interaction proteomics, Biosci. Rep. 25, 45-56, 2005 Zanders, E.D., Ed., Chemical Genomics Reviews and Protocols, Humana Press, Totowa, NJ, 2005 Schou, C. and Heegaard, N.H., Recent applications of affinity interactions in capillary electrophoresis. Electrophoresis 27, 44-59, 2006 Niwayama, S., Proteomics in medicinal chemistry. Mini Rev. Med. Chem. 6, 241-246, 2006 Nedelkov, D. and Nelson, R.W., Eds., New and Emerging Proteomics Techniques, Humana Press, Totowa, NJ, 2006. See also Activity-Based Proteomics. 

While PLP biosynthesis and salvage are now mature research areas, many questions still remain unanswered Many details of the enzymology of the biosynthetic pathways still remain to be resolved and it is not yet clear if all of the biosynthetic or catabolic pathways have been identified. In addition, the PLP transport system in bacteria has not yet been identified and PLP, while covalently attached to its cognate enzyme, has not yet been exploited in activity-based proteomics. 

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