Detection of bacteria and fungi in cell cultures

PROCEDURE DETECTION OF BACTERIA AND FUNGI IN CELL CULTURES 

To examine cell cultures or suspect media for bacterial or fungal contaminants, proceed as follows  

Using an inverted microscope, equipped with phase contrast optics if possible, examine cell culture vessels individually. Scrutiny should be especially rigorous in cases in which large-scale production is involved. Check each culture first using low power. The suppliers listed provide the specific media required but other suitable vendors exist. Batches of media should be tested for optimal growth promotion before use in cell culture quality control. 

After moving the cultures to a suitable isolated area, remove aliquots of fluid from cultures that are suspect and retain these for further examination. Alternatively, autoclave and discard all such cultures. 

Cell and Tissue Culture Laboratory Procedures in Biotechnology, edited by A. Doyle and J.B. Griffiths. 1998 John Wiley Sons Ltd. 

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