Detection and Identification of Bacteria

Bacteria are important biological systems and their association with disease, infection and, recently, bioterror threats, makes their detection of prime interest. Naturally, efforts were made to develop methods of detection based on the vibrational spectroscopy, owing to its high inherent specificity. SERS represents a promising tool for identification and classification of bacteria in clinical microbiology (see Sect. 7.2) or detection of biological warfare agents (see Sect. 4.4). 

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Experiment 24 Detection and Identification of Bacteria via PCR and Subsequent BLAST Analysis of Amplified Sequences... 

Smith, Snyder and Harden (1995) illustrated the profiles of phospholipids (PA, PE, plasmenyl-PE, PC, PG, PS and PI) in extracts of four different bacterial species. They identified both class and molecular weight of the two fatty acyl moieties. They reported different profiles for each bacterium and suggested the possibility of detection and identification of bacteria by ES-MS. 

Karasinski J, White L, Zhang Y, Wang E, Andreescu S, Sadik OA, Lavine B, Vora M (2007) Detection and identification of bacteria using antibiotic susceptibility and a multi-array electrochemical sensm with pattern recognition. Biosens Bioelectron 22 2643-2649... 

Takahashi H, B Kimura, M Yoshikawa, T Fuji (2003) Cloning and sequencing of the histidine decarboxylase genes of Gram-negative, histamine-producing bacteria and their application in detection and identification of these organisms in fish. Appl Environ Microbiol 69 2568-2579. 

LI. Leong, D. E., Loeffelholz, M. J., Purohite, A., and White, T. J., Detection and identification of pathogenic bacteria using the polymerase chain reaction. Abstr. Conf. Nucleic Acids, 5th, 1990, San Diego, Nov. 14-16 (1990). 

A comparison of mutagenic activity using normal and nitroreductase-deficient bacteria provides a method for detection and identification of nitroaromatic mutagens. But this idea is complicated by the existence of several bacterial nitroreductases with differing specificities (11). Three different nitroreductase-deficient derivatives of the normal tester strain TA98 were obtained from Dr H. S. Rosenkranz at Case Western University who has isolated and characterized these strains. Strain TA98NR was selected for its resistance to the nitrocompound niridazole and subsequently found to be resistant to the mutagenicity of niridazole, and also to that of the nitrofurans, nitronaphthalenes, and nitrofluorene (3). It is also resistant to 1-nitropyrene and a number of other nitro-PAH... 

Asano, S., lijima, K., Suzuki, K., Motoyama, Y., Ogata, T., Kitagawa, Y. (2009). Rapid detection and identification of beer-spoilage lactic acid bacteria by microcolony method. Journal of Bioscience and Bioengineering, 108(2), 124—129. 

Rapid detection and identification of spoilage bacteria in beer... 

Kiehne, M., Grdnewald, C., Chevalier, F. (2005). Detection and identification of beer spoilage bacteria using real-time polymerase chain reaction. MBAA Technical Quarterly, 42(3), 214—218. 

Sakamoto, K., Eunahashi, W., Yamashita, H., Masakazu, E. (1997). A rehable method for detection and identification of beer-spoilage bacteria with internal positive control PCR (IPC-PCR). In Proceedings of the 26th European brewery convention congress (pp. 631-638). Maastricht New York, USA Oxford University Press Inc. 

Weber, D. G., Sahm, K., Polen, T., Wendisch, V. R, Antranikian, G. (2008). Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. Journal of Applied Microbiology, 105, 951-962. 

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