Microcolony method

Asano, S., lijima, K., Suzuki, K., Motoyama, Y., Ogata, T., Kitagawa, Y. (2009). Rapid detection and identification of beer-spoilage lactic acid bacteria by microcolony method. Journal of Bioscience and Bioengineering, 108(2), 124—129. 

A rigorous approach to microbiological immersion challenge testing is depicted in Fig. 1. This method uses Pseudomonas diminuta grown in Lactose Broth at 3 rc for 24 h. The culture is then sonicated before use to break up any microcolonies or rosettes that may have arisen and that may reduce the potential for this microorganism to penetrate across tiny flaws in intended microbial barriers. 

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