Deionized water, preparation

Magnesium stock solution—using distilled or deionized water prepare six magnesium standards in 100 ml volumetric flasks of 0.1, 0.3, 0.4, 0.5, 0.6, and 0.8 pg mk concentration from the stock solution. Also prepare 250 ml of a 0.2 pg mpi magnesium standard. 

Prepare solution D by dissolving 25 mg of citric acid in 2.5 mL of deionized water. Add 250 julL of 38% formaldehyde and mix. Bring the final volume to 500 mL with deionized water. Prepare this solution fresh. 

To prepare. Vitamin C standard. Weigh 50 mg of /-ascorbic acid to the nearest 0.1 mg, dissolve in a 50-mL volumetric flask, and dilute to yolume with distilled deionized water. Prepare the day of use. This is a 0.1% solution. Prepare serial dilution of this to obtain also 0.05% and 0.02% standards. 

Deionized water (prepared in this laboratory), glycerol (from BDH), for-mamide (Unilab), and diiodomethane (from Nacalai Tesque) have been chosen as the testing liquids because there are significant data available for these liquids. Table 6.1 tabulates their basic surface tension parameters (in mJ/m ) [45,59]. 

Glutaraldehyde solution 2% (v/v) glutaraldehyde. To make 1 liter of the solution, dilute 20 ml of anhydrous glutaraldehyde to final volume with deionized water. Prepare just prior to use. Store stock glutaraldehyde at 4°C. 

Reducing solution 0.283 M sodium carbonate, 0.007 M formaldehyde. To make 2 liters of the solution, add 60 g of anhydrous sodium carbonate and 1 ml of 37% (w/w) formaldehyde to 1800 ml of deionized water. After dissolving, bring to a final volume with deionized water. Prepare fresh, approximately 30 min prior to use, to allow for the dissolution of the carbonate. 

Prepare individual standards of alanine, arginine, leucine, and valine (Sigma) as 1 pg/pl solutions in deionized water. Prepare each standard by dissolving 10 mg of an amino acid in 10 ml of water. Prepare a mixed standard of the four amino acids at 1 pg/ J.l for each. To obtain the mixed standard, weigh 10 mg of each amino acid and dissolve in 10 ml of water. 

Obtain the following substances DL-lactic acid (85%) DL-malic acid malonic acid-disodium salt monohydrate sodium succinate hexahydrate citric acid monohydrate. Except for the lactic acid, all of these substances are solids at room temperature. Prepare each solid as 5-pg/pl standards in deionized water by dissolving 10 mg of each in 2 ml of deionized water. Prepare lactic acid as a 6-pg/ pi standard as follows Weigh a test tube and then add enough lactic acid with a pipet to increase the weight of the tube by 12 mg. Add 2 ml of deionized water to the tube. This will produce a lactic acid solution of 6 pg/pl. 

Purity of Water—Unless otherwise indicated, references to water shall be understood to mean reagent water as defined by Types II or III of Specification D 1193. Water conforming to the following specification is required. Sul-fate-fiee deionized water prepared by percolation of water through a column of mixed anion and cation exchange resins. 

Solution 71% O-H3PO4 (orthophosphoric add). Mix 5 vol. parts of commercial concentrated (85%) 0-H3PO4 with 1 vol. part of Dl water (deionized water) Preparation Mix 5 vol. parts of (85%) 0-H3PO4 with 1 vol. part glycerin DAB7 and 0.5 —1.0 g/L sodium alkylarylsulfonate (phenysulfonate) 12% NaOH (sodium- hydroxide). Preparation Dissolve 120 g NaOH in 1 L of Dl water 1 % acetic acid. Preparation Add 20 ml glacial acetic acid to 1 L of Dl water... 

Ion exchangers are sometimes used on a throwaway basis also. In the laboratoiy, ion exchangers are used to produce deionized water, purify reagents, and prepare inorganic sols. In medicine, they are used as antacid, for sodium reduction, for the sustained release of drugs, in skin-care preparations, and in toxin removal. 

To a 2-liter flask were added 300 g of finely divided corn. The flask and its contents were then sterilized and after sterilization 150 ml of sterile deionized water wereadded. To the mixture in the flask were then added 45 ml of the inoculum prepared by the process and the material was thoroughly mixed. The mixed material was then incubated for about 20 days at 25°C in a dark room in a water-saturated atmosphere. The following illustrates the recovery of the anabolic substance from the fermentation medium. 

Cyclodextrin solution for extraction studies was prepared at a concentration of 1.00 x 10" iif by dissolving solid y-CDx in deionized water (Continental Water Systems, Atlanta, Georgia). Fresh solution was prepared daily to prevent bacterial growth and CDx decomposition from interfering with complexation and extraction. Cyclodextrin was purchased from Advanced Separation Technologies, Inc. (Whippany, New Jersey) and was used as received. Solid CDx from one lot number was used for all extractions. 

A 20 wt% of Co/TiOa was prepared by the incipient wetness impregnation. A designed amoimt of cobalt nitrate [Co(N03) 6H20] was dissolved in deionized water and then impregnated onto TiOj containing various ratios of rutileianatase obtained from above. The catalyst precursor was dried at 110°C for 12 h and calcined in air at 500°C for 4 h. 

As an electrolyte, Nafion 112 (Du Pont, Inc) membrane was pretreated using H2O2, H2SO4 and deionized water before ion beam bombardment. The prepared membranes with a size of 8 X 8 cm were mounted on a bombardment frame with a window size of 5 x 5 cm, equal to the active area of the test fuel cells, and dried up at 80 C for 2 hr. Then, the mounted membrane was brought in a vacuum chamber equipped with a hollow cathode ion beam source as described in the previous study [1]. Ion dose was measured using a Faraday cup. Ion density... 

CNF supported palladium calalysts Pd/CNFs) were prepared by wet impregnation. CNF was slurtiai in deionized water for about 15 min, and then foe palladium preeursor solution, which contained a desired amotmt of Pd (0.5 wt%), was titi ed to foe slurry. The latter was kept being agitated for a preset period of time, and foea filtaed (without washii and finally dried at about 120 Xi ovemi t. 

The microbial count of the mains water will be reflected in both softened and deionized water which m be prepared from it. 

Deionized water is used in pharmaceutical formulations, for washing containers and plant, and for the preparation of disinfectant solutions. 

Preparation of Emulsions. The entire aqueous phase was stirred until all solids were dissolved. Sufficient water was withheld from the formulation so small volumes of experimental and control components could be added to emulsion subsamples. Sulfuric acid (1 N) was added to the aqueous phase to decrease the pH to 5.7. The two phases in separate containers were blanketed with nitrogen, sealed, and heated to 75 in an 80 water bath (about 30 minutes). The hot oil phase was stirred slowly and blanketed with nitrogen, then the hot aqueous phase was quickly added while stirring. The emulsion was blanketed with nitrogen and slowly stirred (about 2 hours) in the stoppered container until ambient temperature ( 25 ) was reached. Subsamples of the master batch were removed for the addition of experimental components and stored in 1-oz containers. The containers had been washed with hot tap water, deionized water, and methanol, then dried at 120 . 

TA-NaBr-MRNi was prepared by the reported method [3]. RNi (W-1 type) was prepared from 1.9 g of Raney nickel alloy (Kawaken Fine Chemical Co., Ni/Al = 42/58). To wash out the excess base and aluminum salts, a sufficient amount of deionized water was used with ultrasonic irradiation. The modifying solution was prepared by dissolving of (R,R)-tartaric acid (1 g) and NaBr (6 g to 10 g) in 100 ml of water and adjusting the pH to 3.2 with IN NaOH aqueous solution. RNi was heated in the modifying solution at 100 C for 1 hour, washed with water (50 ml), methanol (50 ml, twice), and THF (10 ml). The TA-NaBr-MRNi obtained by this method was immediately used for the hydrogenation. 

Mobile phase The HPLC mobile phase is made up as follows. Prepare 2 L of acetate buffer by dissolving 13.6 g of sodium acetate and 6 mL of glacial acetic acid in 2 L of deionized water. Adjust the solution to pH 4.8 with concentrated sodium hydroxide solution (or glacial acetic acid) if necessary. Mix 2 L of buffer with 1.6-2 L (the amount depends on the particular commodity) of methanol. Eilter the solution through a 0.22-pm Nylon 66 filter membrane before using the mobile phase Absolute ethanol Aaper Alcohol and Chemical Co. (200 proof)... 

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