Inoculum preparation

Superior penicillin producing cultures ate capable of producing in excess of 30 mg/mL of penicillin G (154). Cephalosporin producing strains, however, generally grow poorly and cephalosporin C production is not as efficient as is that of penicillin. Factors such as strain maintenance, strain improvement, fermentation development, inoculum preparation, and fermentation equipment requkements ate discussed in the hterature (3,154). 

Inoculum Preparation Stage Two batches of inoculum of about 50 gallons each are prepared by the following method A 25 ml inoculum (from the germination stage) is transferred to each of four 2-liter flasks, each containing 500 ml of the sterile medium utilized for germination. The flasks and contents are incubated for 5 days at 28°C on a rotary shaker (280 rpm, 2 inch stroke). 

To a 2-liter flask were added 300 g of finely divided corn. The flask and its contents were then sterilized and after sterilization 150 ml of sterile deionized water wereadded. To the mixture in the flask were then added 45 ml of the inoculum prepared by the process and the material was thoroughly mixed. The mixed material was then incubated for about 20 days at 25°C in a dark room in a water-saturated atmosphere. The following illustrates the recovery of the anabolic substance from the fermentation medium. 

Comeau Y, CW Greer, R Samson (1993) Role of inoculum preparation and density on the bioremediation of 2,4-D-contaminated soil by bioaugmentation. Appl Microbiol Biotechnol 38 681-687. 

Table 3 Culture Conditions for Inoculum Preparation and Microbial Recovery...

The most significant running cost (for liquid processes) is inoculum preparation/ medium preparation/fermentation/harvesting/drying, ranging from X to X% of the total running costs. 

Parton, C. and Willis, P. (1990) Strain preservation, inoculum preparation and development, in Fermentation, A Practical Approach (McNeil, B. and Harvey, L. 

Cell Culture Section. The activities in this section include media sterilization, inoculum preparation, cell growth, and virus propagation (Figure 10.1-8). Serum-free (SF) medium is sterilized by 0.2- rm filtration and used for both inoculum preparation and cell growth/virus propagation. The culture is assumed to take... 

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