Data Processing and Reporting

To calculate the concentration, a compound-independent calibration (CIC) is performed using the CLND trace. The relationship between the signal and the concentration is given by the following equation  

Therefore, a calibration curve with caffeine at four different concentrations (0.1, 0.2, 0.4 and 0.8 mM) was constructed. The concentration of the target compound was calculated using Eq. (2)  

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Recently, Chiron introduced the System 340 platform that automates incubation, washing, reading, data processing, and report generation. It is anticipated that automation will dramatically reduce operator-to-operator differences while decreasing labor requirements. In one laboratory the average coefficient of variation was reduced 43% and the hands-on labor was reduced 39% with the automated... 

The high-throughput concept for quantitative bioanalysis applies to steps such as assay development, sample collection and sorting, sample preparation, sample analysis, and data processing and reporting. Those processes are closely interlinked and improvement of process throughput is equally important. 

The titration process has been automated so that batches of samples can be titrated non-manually and the data processed and reported via printouts and screens. One such instrument is the Metrohm 670 titroprocessor. This incorporates a built-in control unit and sample changer so that up to nine samples can be automatically titrated. The 670 titroprocessor offers incremental titrations with variable or constant-volume steps (dynamic or monotonic titration). The measured value transfer in these titrations is either drift controlled (equilibrium titration) or effected after a fixed waiting time pK determinations and fixed end points (e.g. for specified standard procedures) are naturally included. End-point titrations can also be carried out. 

FIGURE 6 A typical sample life cycle from sample injection, through data processing, and reporting.The information finally ends up in the corporate LIMS after final review. 

The computer system attached to the instrument provides instrument control, data acquisition, data processing, and reporting. According to the draft Analytical Instrument Qualification chapter of the United States Pharmacopoeia (USP) <1058> The manufacturer should perform design qualification, validate the software and provide users with a summary of the validation. At the user site, holistic qualification that involves the entire instrument and software system is more efficient than modular validation of the software alone. Therefore the user qualifies the instrument control, data acquisition and processing software by qualifying the instrument. ... 

Many appHcations of chromatography have been automated to some degree attention has focused primarily on the automatic injection of samples or on data processing and reporting. However, the separation power of the column can be usefully exploited as a pretreatment process. 

Data processing and reporting Misinterpretation Data mishandling Data transfer error Unexpected result Inconsistent data pattern... 

Accordingly, the data collection, data processing and reporting are described. 

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