Damaged DNA-binding protein

Tang, J. and Chu, G. (2002) Xeroderma pigmentosum complementation group E and UV-damaged DNA binding protein. DNA Repair, 1, 601-616. Groisman, R., Polanowska, J., Kuraoka,... 

Damaged DNA binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert vnth XPA and replication protein A. J. Biol. Chem. 276, 15434-15440. 

Tomato Tomato/RNAi EH DDBl UV-DAMAGED DNA BINDING PROTEIN 1 TF negatively regulates photomorphogenic responses Higher pigment accumulation due to increase in plastid compartment space [82]... 

Figure 2 Double-stranded oligonucleotide photoprobes that simulate modified DNA and intended to cross-link to DNA-binding proteins. (A) Probe modeling interstrand cross-linking by cisplatin Source From Ref. [63], with permission from the American Chemical Society via the Rightslink service (license number 2458870278307 granted June 30, 2010). The benzophenone probe prior to reaction with DNA is shown in the lower part of the panel. (B) Photoaffinity probe for bacterial DNA repair proteins. TT is a simulated thymine dimer intended to be recognized as a site of damage in DNA, and T (two instances) is the diazirine thymine derivative T Source From Ref. [64], with permission from Wiley.

Study of other rare hereditary cancers has led to the location of 20 or more additional probable tumor-suppressor genes. One of these, p53, is inactive in over 50% of all human cancers and over 90% of sqamous cell carcinomas of the skin.ee In small-cell lung cancers and osteosarcoma both RB and p53 are inactive.2 Protein p53 is a stronger tumor suppressor than protein Rb. Results of a variety of experiments have suggested that p53, a DNA-binding protein of known structure, plays a key role in checking DNA for damage at the C,... 

Specific DNA-protein interactions which either promote or inhibit CT processes through the protein-DNA interface would be the most crucial part of a biological system sensing DNA damage. Recent experiments have shown clearly that DNA-mediated CT processes are modulated both negatively and positively by DNA-binding proteins. Most importantly, each of the observed influences of the proteins can be explained by special structural features of the corresponding DNA-protein complexes. Thus, special DNA-protein interactions result in a characteristic modulation of the DNA-mediated CT. 

Barton et al. developed an assay enabling the study of the influence of DNA-binding proteins simply by gel electrophoretic analysis of oxidative G damage... 

Rajski SR, Barton JK. How different DNA-binding proteins affect long-range oxidative damage to DNA Biochemistry 2001 40 5556-64. 

Another protein able to sequester iron in a mineral core is Dps, a member of the ferritin superfamily. The name Dps comes from DNA-binding protein from starved cells, but although some Dps (for example, those from E. coli and Synechoccus sp. ) do bind DNA nonspecifically, others such as those from Campylobacter jejuni and Agrobacterium tumefaciens have no DNA-binding activity. These proteins retain the ability to protect DNA from damage via reactions with hydrogen peroxide. 

Brody. X, Keeney, S., and Linn, S. (1995). Human damage peciftc DNA binding protein p40 subunit mRNA. Genbank, Accession 18299. 

A. (2005) Xeroderma pigmentosum complementation group E protein (XPE/DDB2) purification of various complexes of XPE and analyses of their damaged DNA binding and putative DNA repair properties. Mol. Cell. Biol., 25, 9784-9792. 

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