Replication protein A

Bochkarev, A., Pfuetzner, R. A., Edwards, A. M., and Frappier, L. (1997). Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature 85, 176-181. 

Hovanessian AG, Puvion-Dutilleul F, Nisole S, Svab J, Perret E, Deng JS, Krust B (2000) The cell-surface-expressed nucleolin is associated with the actin cytoskeleton. Exp Cell Res 261 312—328 Iftode C, Daniely Y, Borowiec JA (1999) Replication protein A (RPA) the eukaryotic SSB. Crit Rev Biochem Mol Biol 34 141-180... 

Two other protein complexes also function in eukaryotic DNA replication. RPA (replication protein A) is a eukaryotic single-stranded DNA-binding protein, equivalent in function to the E. coli SSB protein. RFC (replication/actor (J) is a clamp loader for PCNA and facilitates the assembly of active replication complexes. The subunits of the RFC complex have significant sequence similarity to the subunits of the bacterial clamploading (y) complex. 

Cells of patients with Bloom syndrome (BS) have many chromosome breaks and a high frequency of sister chromatid exchanges, perhaps in an effort to correct these breaks. The body is small but well-proportioned.kk A somewhat similar disease, the Werner syndrome (WS), is associated with premature aging.11 The Bloom s protein BLM and the WS gene product WRN are both helicases related to E.coli RecQ. Protein BLM colocalizes with replication protein A as discrete foci in the meiotic synaptonemal complex.1 3 Protein WRN also seems to be associated with DNA replication. Defects... 

Human replication protein A Replication ILIO CysL 2 Cysp 13 Cysp 4 Cysp (N)... 

Walter J, Newport J. Initiation of eukaryotic DNA replication origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. Mol. Cell 2000 5 617-627. Zou L, Stillman B. Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled... 

Zou L, Liu D, Elledge SJ. Replication protein A-mediated recruitment and activation of Radl7 complexes. Proc. Natl. Acad. Sci. 168. U.S.A. 2003 100 13827-13832. 

Lao Y, Gomes XV, Ren Y, Taylor IS, Wold MS. Replication protein A interactions with DNA. III. Molecular basis of recognition 169. of damaged DNA. Biochemistry 2000 39 850-859. 

Replication protein A (RPA), a protein complex with affinity to ssDNA, is a key player in many types of DNA metabolisms such as DNA replication and DNA repair (40). In G2 yeast cells, depletion of RPA abolished the localization of Ddc2 to HO-induced DSBs (41), suggesting that RPA is important for... 

Wold MS. Replication protein A a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Ann. Rev. Biochem. 1997 66 61-92. 

DNA helicases separate the parental DNA strands, and the single strands are stabilized by the binding of replication protein A, a single-stranded- DNA-binding protein. Replication begins with the binding of DNA polymerase a, which is the initiator polymerase. This enzyme has primase activity, used to synthesize RNA primers, as well as DNA polymerase activity, although it possesses no exonuclease activity. After a stretch of about 20 deoxynucleotides have been added to... 

Li, R. and Botchan, M. R. (1993) The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication. Cell 73, 1207-1221. 

Nucleotide-excision repair involves PCNA, DNA polymerase epsilon, and several accessory proteins, such as RF-C (replication factor C), RP-A (replication protein A), and Lig I (DNA ligase I). It could be that these proteins form an aggregate DNA repair machine. PCNA is a hollow circular protein that encircles DNA. PCNA binds and activates many proteins involved in DNA repair. Mismatch repair is less well characterized, but involves DNA polymerase delta. 

Sugasawa, K., Weterings, E., Jaspers, N.G., and Hoeijmakers, J.H. (1998) DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair. Genes Dev., 12, 2598-2609. 

The two subpathways of NER, global genome (GG)-NER [8] and transcription-coupled (TC)-NER [9], employ a common set of proteins including XPC/HR23B (also known as XPC-RAD23B Chapter 11), TFIIH, XPA, RPA (replication protein A), XPG, and ERCC1 (excision repair cross-complementation group 1)/XPF. The... 

RPA Replication protein A Inhibition by protein binding Replication/DNA Repair Dutta etal. (1993)... 

After parental DNA Is separated into single-stranded templates at the replication fork, it is bound by multiple copies of RPA (replication protein A), a heterotrimeric protein (Figure 4-34c). Binding of RPA maintains the template in a uniform conformation optimal for copying by DNA polymerases. Bound RPA proteins are dislodged from the parental strands by Pol a and Pol 8 as they synthesize the complementary strands base-paired with the parental strands. 

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