Culture media preparation

Basic equipment for plant culture media preparation and sterilization, controlled growth chamber, microscope, stereomicroscope and inverted microscope equipped with a photocamera, laminar flow cabinet. 

As shown in Fig. 15.17, Kim et al. studied various cathode catalysts for oxygen reduction in microbial fuel cells that contained culture media prepared with 1 g sodium acetate solution in 50 mM phosphate buffer containing 12.5 mL mineral solution and 5 mL L vitamin solution [95]. Carbon-supported FePc showed similar ORR activity as the carbon powder, which had more than... 

The spores from an inclined culture of Fusarium lateritium Wr, CSB 119.63 on a gelose medium are extracted with sterilized distilled water to obtain a suspension containing about 600,000 spores per ml. This suspension is then used to seed the medium prepared as earlier described. The contents of the flask are left to incubate at 27°C. Sterile air is injected into the liquid to effect thorough agitation and uniform supply of oxygen into the medium. 

The culture is incubated at a temperature of 28°C in the reactor for 60 hours with mechanicai agitation and constant aeration. The resulting broth is seeded into 600 liters of a sterile culture medium contained in a metal fermenting vat 1,800 liters in capacity and prepared according to the following formulation ... 

A small fermentation tank (5,000 parts by volume capacity) was charged with 3,000 parts by volume of a culture medium (pH 6.0) comprising 3% glucose, 1 % polypepton, 0.5% yeast extract and 0.5% malt extract. The medium was sterilized by heating in a conventional manner and cooled. This medium was inoculated with 150 parts by volume of a pre[Pg.1565]

Product extraction Effluent and waste disposal Medium preparation Seed vessel Purification Cell free supernatant Cell biomass Production bioreactor Downstream processing Medium sterilisation Primary culture Upstream processing... 

An improved method of producing recombinant aequorin was devised based on the fact that the expression of the peak amount of apoaequorin in bacterial cells occurs several hours before the secretion into culture medium (Shimomura and Inouye, 1999). The cells containing apoaequorin in the periplasmic space, before secretion, are extracted under a very mild condition and, at the same time, converted into aequorin. The purification of the extract by two steps of column chromatography yields a high-purity preparation of recombinant aequorin. 

Biological indicators (Bis) for use in thermal, chemical or radiation sterilization processes consist of standardized bacterial spore preparations which are usually in the form either of suspensions in water or culture medium or of spores dried on paper, aluminium or plastic carriers. As with chentical indicators, they are usually placed in dummy packs located at strategic sites in the sterilizer. Alternatively, for gaseous sterihzation these may also be placed within a tubular hehx (Line-Pickerill) device. After the sterilization process, the aqueous suspensions or spores on carriers are aseptically transferred to an appropriate nutrient medium which is then incubated and periodically examined for signs of growth. Spores of Bacillus stearothermophilus in sealed ampoules of cultrrre medium are used for steam sterilization morritoring, and these may be incubated directly at 55°C this eliminates the need for an aseptic transfer. 

Keeping lycopene soluble and unoxidized in the warm, aqueous tissue culture medium over the incubation periods of 12-72 h is problematic. Furthermore, depending on the lycopene preparation, variable amounts remain on the filters used to prevent microbial contamination and hence the... 

In the approach followed in this invention [29], a biocatalytic agent converts the sulfur heterocycles into different molecules that do not exhibit the hydrophobic interactions. This is achieved by selectively cleaving carbon-sulfur bonds. The selectivity of the biocatalytic agent employed is limited to the carbon-sulfur bonds and no attack to the carbon-carbon skeleton was reported. Thus, it is expected that the proposed biocatalytic reduction of viscosity would not diminish the fuel value of the treated petroleum liquids. The biocatalyst employed consisted of the strain ATCC No. 53968 (see Section 20 and references therein), in an aqueous culture conventionally prepared by fermentation under aerobic conditions. The fermenting bioreactor is fed with a suitable nutrient medium, which comprises a conventional carbon source (dextrose and glycerol are recommended carbon sources. To confer maximal biocatalytic activity for the desired cleavage of organic C—S bonds, the bacteria was kept in a state of sulfur deprivation. 

One of the most sensitive biological effects of ionizing radiation is to increase the frequency of normally observed chromosome aberrations (but not to induce qualitatively special abnormalities). Peripheral blood lymphocytes are the most feasible cells for chromosome investigations, as blood samples are easy to obtain and the techniques to stimulate the lymphocytes to proliferate within a culture medium and to prepare suitable chromosome slides for microscopic analyzation have their routine protocoil (e. g. Yunis, 1965 Lloyd et al, 1982). 

Prepare also a blank containing no allelochemical in the culture medium... 

There have been several studies that underscore the importance of unbound concentration in cell-based studies of receptor function. In a model study of the effect of plasma protein binding on the renal transport of organic anions using the expression of various organic anion transporters (OATPs) in Xenopus oocytes, the transport of ochratoxin A, methotrexate, and estrone sulfate was found to be strongly inhibited by the addition of human serum albumin to the culture medium [16]. Similarly, the addition of oq-acid glycoprotein was found to reverse the blockade of sodium-ion current by cocaine in a preparation of cardiac myocytes [17]. 

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