ATCC 367 strain

Living or heat-killed bacteria Gram-positive and Gram-negative ATCC strains. 

M. J. Edwards, Microbes and Cells at Work An Index to ATCC Strains with Special Applications. American Type Culture Collection, 12301 Parklawn Drive, Rockville, Maryland, 1988. 

Challenge Microorganisms. The antimicrobial spectrum of activity of the test products must be broad, versus 25 ATCC strains of 20 species of bacteria and 2 species of yeasts listed in 333.470(a)(l)(ii) of the TFM, plus 25 fresh clinical isolates of these same species of bacteria and yeasts. The challenge microorganisms include members of normal flora in humans, common environmental contaminants, or systemic pathogens. 

Mice were inoculated i.v. with pyran and challenged i.v. 24 hours later with 50 LDso s of encephalomyocarditis (EMC) virus. The EMC virus (ATCC strain VR129) was prepared as a 10% brain homogenate from infected weanling mice. Deaths were recorded daily and percent mortality calculated (Morahan, Regelson, and Munson, 1972 Morahan et al, 1972). 

The antibacterial activity of the essential oil of Lippia multiflora was rst examined in vitro for antimicrobial activity against American type culture collection (ATCC) strains and clinical isolates of the buccal ora. A signi cant activity was found, with an minimum bactericidal concentration (MBC) of 1/1400 for streptococci and staphylococci, 1/800 for enterobacteria and neisseria, and 1/600 for Candida. A mouthwash was prepared with the essential oil at a 1/500 dilution, and this was used in two clinical trials. 

The antibacterial activity of the essential oil of Lippia multiflora was first examined in vitro for antimicrobial activity against ATCC strains and clinical isolates of the buccal flora. A significant... 

Figure 1. Gas Chromatograms of Slaframine Standard and Extracts of Wisconsin, North Carolina, and ATCC Strains. Standard and Strain Extracts Vary in SF Concentration.

Welan Gum. This gum is produced by a carefiiUy controUed aerobic fermentation using 2nc. ydlcaligenes strain (ATCC 31555) (88). The backbone... 

Rhamsan Gum. Rhamsan gum, produced y lcaligenes strain (ATCC 31961) (91), has the same backbone as geUan and welan gums, but it carries a disaccharide sidechain (92). 

The following process description is taken from U.S. Patent 2,987,449. An appropriate S. aureofaciens strain such as mutant S1308 (ATCC No. 12,748) is grown aerobically in a suitable inoculum medium. A typical medium used to grow the primary inoculum is prepared according to the following formula sucrose, 20.0 g corn steep liquor, 16.5 ml ammonium sulfate, 2.0 g calcium carbonate, 7.0 g and water to 1,000 ml. 

A 100 ml aliquot of this medium is placed In a 500 ml Erlenmeyer flask and sterilized by autoclaving for 20 minutes under 15 psi pressure. Spores of mutant strain S. aureofaciens S1308 (ATCC No. 12,748) are washed from an agar slant Into the flask with sterile distilled water to form a suspension containing approximately 10 spores per milliliter. A 1.0 ml portion of this suspension is used to inoculate the fermentation media in the example which follows. A fermentation medium consisting of the following ingredients was prepared. 

This material was made up with distilled water to provide 41 g per liter, and the mixture was adjusted to pH 7.0 with potassium hydroxide solution. To the mixture were added per liter 5.0 g of calcium carbonate and 7.5 ml of soybean oil. 2,000 ml portions of this medium were then added to fermentation vessels, equipped with stirrers and aeration spargers, and sterilized at 121°C for 60 minutes. After cooling the flasks were inoculated with a suspension of strain No. ATCC 11924 of Streptomyces lavendulae, obtained from the surface of agar slants. The flasks were stirred for 4 days at 28°C at approximately 1,700 rpm. At the end of this period the broth was found to contain cycloserine in the amount of about 250 C.D.U./ml of broth. The mycelium was separated from the broth by filtration. The broth had a pH of about 7.5. Tests showed it to be highly active against a variety of microorganisms. 

A. Culturing of MK-65 In this example, Micromonospora sagamiensis MK-65 ATCC 21826 (FERM-P No. 1530) is used as the seed strain. One loopful of the seed strain is inoculated into 30 ml of a first seed medium in a 250 ml-Erlenmeyer flask. The first seed medium has ... 

The SF-837 strain, namely Streptomyces mycarofaciens identified as ATCC No. 21454 was inoculated to 60 liters of a liquid culture medium containing 2.5% seccharified starch, 4% soluble vegetable protein, 0.3% potassium chloride and 0.3% calcium carbonate at pH 7.0, and then stir-cultured in a jar-fermenter at 28°C for 35 hours under aeration. The resulting culture was filtered directly and the filter cake comprising the mycelium cake was washed with dilute hydrochloric acid. 

Citric acid fermentation of cane-molasses by submerged fermentation in 21 B. Braun stirred fermenter (working volume 21) is performed. A strain of A. niger ATCC 11414 is... 

Schenk T, R Muller, F Lingens (1990) Mechanism of enzymatic dehalogenation of pentachlorophenol by Arthrobacter sp. strain ATCC 33790. J Bacterial 172 7272-7274. 

Bhushan B, A Halasz, J Spain, J Hawari (2004) Initial reaction(s) in biotransformation of CL-20 is catalyzed by salicylate 1-monooxygenase from Pseudomonas sp strain ATCC 29352. Appl Environ Microbiol 70 4040-4047. 

Topp E, L Xun, CS Orser (1992) Biodegradation of the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzo-nitrile) by purified pentachlorophenol hydroxylase and whole cells of Flavobacterium sp. strain ATCC 39723 is accompanied by cyanogenesis. Appl Environ Microbiol 58 502-506. 

Uetz T, R Schneider, M Snozzi, T Egli (1992) Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from Chelatobacter strain ATCC 29600. J Bacteriol 174 1179-1188. 

Lee J-Y, L Xun (1997) Purification and characterization of 2,6-dichloro-p-hydroquinone chlorohydrolase from Flavobacterium sp. strain ATCC 39723. J Bacterial 179 1521-1524. 

Oldfield C, O Pogrebinsky, J Simmonds, ES Olson, CE Kulpa (1997) Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968). Microbiology (UK) 143 2961-2973. 

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