Video enhanced contrast

Video-Enhanced Contrast. This technique is more expensive but much more effective than any other contrast-enhancing technique (15). Since the 1970s, the development of video processing of microscopical images has resulted in electronic control of contrast. As Shinya InouH, author of a classic text in the field, states "We can now see objects that are far too thin to be resolved, and extract clear images from scenes that appeared too fuzzy, too pale, or too dim, or that appeared to be nothing but noise" (16). The depth of the in-focus field can now be expanded or confined, very thin but very sharp optical sections can be produced, and a vertical succession of these images can be accumulated to reconstmct thicker stmctures in three dimensions (16). 

Shooton, D. (ed.) (1993) Electronic Light Microscopy. The Principles and Practice of Video-Enhanced Contrast, Digital Intensified Fluorescence, and Confocal Scanning Light Microscopy. Wiley-Liss, New York. 

While even relatively simple techniques can dramatically improve contrast ( ), perhaps it is the exotic new methods, such as those for producing "live" stereoscopic images at near-micron resolution (49.501. or the Allen Video Enhanced Contrast system (44.451. which typify the explosive growth in the zuicient science of visible microscopy. Although the focus has been on biosystems, there is no reason why these technologies cannot be... 

Video microscopy has permitted direct observation of microtubule assembly/disassembly dynamics in vitro. Horio and HotanP first used dark-field optics to observe the growth and shrinkage phases, but so-called Allen video-enhanced contrast microscopy has become most convenient. 

Masters BR, Kino GS. Charge coupled devices for quantitative Nipkow Disk real-time scanning confocal microscopy. In Shotton D, ed. Electron light microscopy The principles and practice of video-enhanced contrast, digital intensified fluorescence, and confocal scanning light microscopy. New York Wiley-Liss, 1993. 

Shotton D. Electronic hght microscopy the principles and practice of video- enhanced contrast, digital intestified flourescenes, and confocal scanning hght microscopy. Wiley-Liss 1992, pp. 17-20 (Chap 1). 

R. D. Allen el al. "Video-Enhanced-Contrast Polarization (AVEC-Pol) Microscopy A New Method Applied to the Detection of Birefringence in the Motile Retic-ulopodial Network of Allogromia Lalicollaris. Cell Motil. 1 (1981) 275-289. 

Bruckner, E. and Rehage, H., Solubilization of toluene in phospholipid vesicles studied by video-enhanced contrast microscopy, Progr. Colloid Polym. ScL, 109, 21-28 (1998). 

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