Colonic cell lines butyrate

The concentration of sodium butyrate was observed to have a differential effect on cell growth in colonic cell lines. After culturing for 8 days with 5 mM sodium butyrate, the cell protein per flask of the SCKO-1 line was decreased to less than 10% of the control cultures. In the SW-620 culture,cell protein per... 

AVIVI-GREEN c, PLOAK-CHARCON s, MADAR z, SCHWARTZ B (2002) Different molecule events account for butyrate-induced apoptosis in tiw human colon cancer cell line. J. Nutr. 132 1812-18. 

HAGUE A, MANNING A M, HANLON K A, HUSCHTSCHA L I, HART D, PARASKEVA C (1993) Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway impUcations for the possible role of dietary fibre in the prevention of large-bowel cancer. /ni J Cancer. 55 498-505. 

Nakano K, Mizuno T, Sowa Y, Orita T, Yoshino T, Okuyama Y, Fujita T, Ohtani-Fujita N, Matsukawa Y, Tokino T, Yamagishi H, Oka T, Nomura H, Sakai T. (1997) Butyrate activates the WAFl/Cipl gene promoter through Spl sites in a p53-negative human colon cancer cell line. J Biol Chem 212 22199-22206. 

This study describes the effect of sodium butyrate on glyco-lipids from four human colonic tumor cell lines, SKCO-1, HT-29, SW-480 and SW-620 and a human fetal intestinal line, FHS. 

In the present study, we examined the pattern of fucolipids and gangliosides in cultured cell lines and alterations produced by a differentiating agent. A human fetal intestinal line (FHS), and four human colonic tumor lines (SKCO-1, HT-29, SW-480 and SW-620) were used. Cells were grown with or without sodium butyrate, (1.0 mM in SKCO-1 and 2.5 mM in all other cell lines) in... 

Increased fiber intake and the consumption of fruits and vegetables are well-known chemopreventive dietary strategies against colon carcinogenesis. It is reported that fiber intake reduces colonic transit time and increases the production of short chain fatty acids such as butyric acid. The latter is produced in the mammalian colon in mM concentrations as a by-product of anaerobic bacterial fermentation of dietary fiber. Butyric acid has been recognized as one candidates chemopreventive active against colon carcinogenesis because it induces apoptosis and differentiation in colon cancer cell lines. 

---