Coatings pullulan

Crang, R. E. Pechak, D. G. (1978). Aureobasidium pullulans fine structure and development. Journal of Coatings Technology, 50, 36-42. 

The relative mildew growth on polymer and on paint samples exposed to Aurobasldlum pullulans was also studied using the "Zabel Test." In this procedure, veneer strips of southern yellow pine and bass wood are sterilized, cooled, and painted (2 coats with 24 hrs. drying time). After 2-3 days the strips were soaked In distilled water, sterilized at 100 C and placed vertically In 16-ounce French square bottles containing 20 gm. of vermiculite. A mlcronutrlent enriched nutrient media was then poured Into the vermiculite (100 ml per bottle). After sterilization at 121 the painted strips were positioned so that the bottom of the painted zone was 0.5 cm above the vermiculite surface. The specimens were Inoculated with a spore suspension of Aureobasldlum pullulans (16624) by pipette. Incubated two months at 28 , and then rated visually with a 20x binocular. In the weathered series, the specimens were exposed four days In an Atlas Weather 0 Meter (UV and water spray). Representative results are summarized In Table 13. 

A standard Paint Research Institute latex paint, provided by J. F. Matthews of DuPont, was the reference sample to which the biocidal latex polymers were compared. After two months both the unweathered and weathered samples showed moderate or moderate to heavy growth on the paint surface. The unweathered samples showed severe stain intensities on basswood. The weathered samples showed medium stain intensities on both. When coatings of an MMA/nBA latex blended with a MMA/nBA/8 terpolymer latex were tested, only trace growth of Aureobasidlum pullulans on the coatings was observed using pine or basswood substrates. 

Schmitt, J. A., "Growth Patterns of Paint Mildew and Aureobasidium pullulans," Journal of Coatings Technology, 50,... 

Mildew defacement of organic coatings and plastics has long been a major problem of the organic coatings and plastics industries. Aureobasidium pullulans is the major causitive organism... 

Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis (SDS-CGE). SDS-CGE is a capillary-based version of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the slab gel format, with advantages of shorter analysis times, ease of automation, and online detection and quantitation [26, 27]. In SDS-CGE, replaceable sieving polymers, such as linear polyacrylamide, poly (ethylene oxide), dextran, or pullulan, are used to achieve reproducible separations. These polymers permit the replacement of a separation matrix for each sample, thereby eliminating cross-contamination between samples and improving reproducibility. Best results are often obtained using chemically or dynamically coated capillaries. 

Pullulan films have several other advantages. The films are colorless, tasteless, odorless, transparent, resistant to oil and grease, and heat sealable. The properties of the films can be modified by chemical modification of pullulan, blending with poly-vinylalcohol, gelatin, or amylose, and by addition of plasticizers. These properties indicate that pullulan can be used as a coating or packaging for foods to prevent their oxidation. This is the only apparent outstanding application for pullulan. 

Iwamoto et al. suggested that the tissue distribution of an O/W emulsion can be controlled by coating their surfaces with a cell-specific cholesterol-bearing polysaccharide, such as pullulan, amylopectin, and mannan (10,24). 

Coating of emulsion particles with cholesterol-bearing polysaccharides, such as pullulan, amylopectin, and mannan, led to a reduction of the divalent metal ion-induced aggregation of Che emulsion droplets. The cholesteryl group behaves as a hydrophobic anchor in the surface lipid layer resulting in a lower fluidity of the. surface of the emulsion droplets (24). 

Hydrolytic digestion of pullulan with pullulanase and subsequent release of FITC moiety from the surface of liposomes were examined by a decrease in the steady-state fluorescence depolarization. Measurements were run on a Union Giken fluorescence polarization spectrophotometer FS-501S, where the FITC fluorescence (520 nm) was detected by exciting the sample at 440 nm using a sharp cut-off filter Y46 (Hoya Glass Works, Tokyo). To a 3.0 ml solution of liposome (1.0 xio" M as egg PC) coated with a given amount of FITC(0.54)-OPP-50(1.8) was added 20 yl of pullulanase solution (2.0 unit) after preincubation for 5 min in 200 mM Tris-HCl (pH 8.0) at 25.0 °C. 

The fourth investigation was the enzymatic digestion of FITC-OPP bound to liposomes with pullulanase.The steady-state fluorescence depolarization (p) about the FITC(0.54)-OPP-50(1.8)-coated liposomes showed the restriction of mobility of polysaccharides, where the higher p-value were obtained for FITC-OPP with liposomes than for free FITC-OPP without liposomes (Fig. 5). When pullulan was digested by pullulanase, which is the enzyme to cleave specifically the al->6 linkage of pullulan (see Fig. 1), a drastic decrease in the p-value was observed. This is caused by gains of the... 

Pullulan Film-forming agent Thickener Capsules for dietary supplements Coatings for coated tablets Wound-healing component 121,122... 

Zhao, L. Tang, J. (2010). Pullulan acetate coated magnetite nanojjarticles for hyper-thermia Preparation, characterization and in vitro experiments. Nano Res., 3, 23-31. 

The [Gj] structure (where represents the a-l,4-linked trimer maltotriose and n the number of maltotriose units), together with the presence of hydroxyl groups along the chain, confer pullulan its unique physicochemical properties very good solubility in water and high structural flexibility. Pullulan capacity to form thin layers, electros-pxm nanofibers, nanoparticles, flexible coatings or stand-alone films [115] makes it very attractive for various pharmaceutical and medicinal applications. 

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