Hoya glasses

Hydrolytic digestion of pullulan with pullulanase and subsequent release of FITC moiety from the surface of liposomes were examined by a decrease in the steady-state fluorescence depolarization. Measurements were run on a Union Giken fluorescence polarization spectrophotometer FS-501S, where the FITC fluorescence (520 nm) was detected by exciting the sample at 440 nm using a sharp cut-off filter Y46 (Hoya Glass Works, Tokyo). To a 3.0 ml solution of liposome (1.0 xio" M as egg PC) coated with a given amount of FITC(0.54)-OPP-50(1.8) was added 20 yl of pullulanase solution (2.0 unit) after preincubation for 5 min in 200 mM Tris-HCl (pH 8.0) at 25.0 °C. 

Table 3.4-14 gives an overview of the prefened optical glasses from Schott, Hoya, and Ohara. The glass types are listed in order of increasing refractive... 

Hoya, Photo sensitive Glass PEG 3. Technical Documentation. Hoya Corporation, Electro-Optics Department, Tokyo, Japan, 1994... 

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