Coagulation activation

A proteolytic cascade occurs when one peptidase activates the next in a proteolytic pathway, and this in turn activates the next and so on. This is a mechanism to amplify the initial signal, because one peptidase molecule can activate many zymogen molecules. Examples of proteolytic cascades include blood coagulation, activation of digestive peptidases in the intestine, and apoptosis. 

MARCKMANN p, sandstrOm b, jespersen J (1990) Effect of total fat content and fatty acid composition in diet on factor Vll coagulant activity and blood lipids, Atherosclerosis, 80, 227-33. 

Merlini P. A., Ardissino D. Current status of activation markers in ischemic heart disease Markers of coagulation activation. Thromb Haemost 1997 78,276-9. 

DETA/NO is a stable NO-donor with the longest NO generation half-life of approximately 20 h. Thrombelastography performed on rabbit blood showed that DETA NONOate-derived NO significantly decreased coagulation activity and platelet activation [48]. Monitoring by intravital microscopy showed that DETA/NO attenuated the platelets/endothelial cells adhesion response to endotoxins (e.g. lipopolysaccharides) in murine intestinal venules [49]. The main mechanism of the antiadhesive action of DETA/NO on platelets was activation of soluble guanylate cyclase [49]. 

Hemophilia A Determine factor VIII coagulant activity before injecting desmopressin for hemostasis if activity is <5% of normal, do not rely on desmopressin... 

Synthetic heparinoid polymers such as polyanionic polyesters containing sulfamate and carboxylate groups were synthesized by hydrolysis of p(N chloro-sulfonyl beta lactam) [475]. These materials possess anti-coagulant activity (although lower than heparin) presumably due to the similarity of functional groups of this material and those found in heparin. 

Affects coagulant activity during coagulation and the retention of active coagulant in the curd. 

Diokno-Palo, N., Palo, M. A., Cunanan, L. F. and Santos, P. S. 1979. Skim milk-coagulating activities of enzymes produced by phycomycetous fungi. Philippine J. Sci. 108, 137-151. 

Nachev, L., Velcheva, P. and Kolev, D. A. 1973. Bacterial enzyme complex with milk-coagulating activity. I. Preparation and some properties. Prilozh. Mikrobiol. 1, 31-37. 

Sandberg H, Bode AB Dombrose FA, Hoechli M, Lentz BR. Expression of coagulant activity in human platelets release of membranous vesicles providing platelet factor I and platelet factor 3. Thromb Res 1985 39 63-79. 

Numerous observational studies have found that vitamin C may decrease LDL cholesterol and elevate HDL (50). Some studies found an inverse association between serum vitamin C concentration and coagulation factor or coagulation activation markers (51). 

Christensen K, Larsson A, Emanuelsson H, et al. The stent graft modulation of platelet and coagulation activation with heparin coating [abstr], Eur Heart J 1998 19 (suppl) 498. 

Hietala EM, Maasilta R Valimaa T et al. Platelet responses and coagulation activation on polylactide and heparin-polycaprolactone-L-lactide-coated polylactide stent struts. J Biomed Mater Res A2003 67(3) 785—791. 

The changes in eluting buffer are made after the column has equilibrated with the buffer. The protein fractions obtained in this way are assayed for coagulant activity. 

Less than 1% of the applied coagulant activity is recovered in fraction Nos. 1, 2, 3, 4. Fraction 1 however possesses proteolytic activtity which in concentrated solutions would dissolve fibrin clots. The thrombin-like activity is eluted from these columns in significant amounts at a buffer strength of 0.04 M or greater. 

Factor IX coagulant activities (APTT) +/+ 92%, +/- 53 %, -/- <5% bleeding disorder (extensive bleeding after clipping a portion of the tail, bleeding to death if not cauterized (Kundu et al. 1998 Wang et al. 

Healy AM, Hancock WW, Christie PD et al. (1998) Intravascular coagulation activation in a murine model of thrombomodulin deficiency effects of lesion size, age, and hypoxia on fibrin deposition. Blood 92 4188-4197 Weiler-Guettler H, Christie PD, Beeler DL et al. (1998) A targeted point mutation in thrombomodulin generates viable mice with a prethrombotic state. J Clin Invest 101 1983-1991... 

Figures 6 and 7 show the anti-coagulant activity expressed in international units (IU)/mg, as determined by the modified Commentary of the Japanese Pharmacopoeia M and the acute toxicity LD50 (when anti-coagulant is injected into the vein of a rat), as functions of f2> + f3 ) and Mn 24). The solid lines represent the contours of the same(Fig. 6) and LD50 (Fig. 7). Larger ( f2 + f3 ) results in larger % when compared at the same Mn. For a fixed ( -1- % tends to increase with...

There are close relationships between the distribution of the degree of substitution fk>, physical properties of cellulose derivatives (CD) we are presently approaching the goal to design the particular molecular structure of CD required for a desired physical property (for example, anti-coagulant activity or solvent absorbing power). 

Lox C. 1995. Alterations in coagulant activity following exposure to industrial solvents a possible marker of toxic chemical induced pathogenesis. Keystone Symposium On Molecular Toxicology, Copper Mountain, Colorado, USA, January 9-15, 1995. Journal Of Cellular Biochemistry Supplement 0 (19a) 197. 

---