Chromatography method development

Considerable background information on chromatography and mass spectrometry, for the purposes discussed in this book, is provided in Chapters 3-7. As emphasized previously it is not a trivial task to sort through all the numerous combinations of available technologies (Hopfgartner 2003a) to decide on an optimum method for the purpose at hand. The present section describes some practical approaches to development of a chromatographic method that is fit for purpose. [Pg.507]

In this context it is helpful to bear in mind three major objectives (i) the chromatographic method should be sufficiently fast to meet requirements for sample throughput (determined by number of samples and any deadlines set by the client) (ii) if a value of LLOQ has been specified, during development of a chromatographic method it is advisable to aim for a signakbackground ratio 10 at this LLOQ concentration whenever possible (iii) the method should be rugged and reliable in the presence of matrix, i.e. analyte in extracts of control matrix. [Pg.507]

R. -S. Tsai, P.-A. Carrupt, and B. Testa, Measurement of Partition Coefficients Using Centrifugal Partition Chromatography. Method Development and Application to the Determination of Solute Properties, American Chemical Society, Washington DC, 1995, pp. 143-154. [Pg.765]

L. van Heukelem and C.S. Thomas, Computer assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments. J. Chromatogr.A 910 (2001) 31—49. [Pg.363]

High-pressure liquid chromatography method development of pharmaceuticals is an iterative process required to support successive phases of pharmaceutical development and clinical studies. This chapter details the approach currently in use in our laboratories, from receipt of a new chemical entity to post transfer support. [Pg.145]

An Expert System for High Performance Liquid Chromatography Methods Development... [Pg.278]

Kang, J. W., Van Schepdael, A., Roets, E., and Hoogmartens, J. (2001). Analysis of vancomycin and related impurities by micellar electrokinetic capillary chromatography. Method development and validation. Electrophoresis 22, 2588—2592. [Pg.311]

Roston, D.A. Sun, J.J. Collins, P.W. Perkins, W.E. Tremont, S.J. Supercritical fluid extraction-liquid chromatography method development for a polymeric controlled-release drug formulation. J. Pharm. Biomed. Anal. 1995, 13 (12), 1513-1520. [Pg.3581]

A method which has proved to be exceedingly useful is an extension of the partition chromatography method developed by Martin and Synge (page 164), which was first applied by James and iMartin to the separation of volatile fatty acids. The method has subsequently been... [Pg.166]

Fig 13. Final polishing step of recombinant epidermal growth factor, using reversed phase chromatography. Method developed on pre-packed RESOURCE RPC and scaled up on... [Pg.41]

Tsai, R.-S., Carrupt, P.-A. and Testa, B. (1995) Measurement of partition coefficient using centrifugal partition chromatography. Method development and application to the determination of solute properties, in Modern Countercurrent Chromatography (eds W.D. Conway and R.J. Petroski), American Chemical Society, New York, pp. 143-154. [Pg.1187]

Grogg-Sulser, K. Helmlin, H.-J. Clerc, J.-T. Qualitative and quantitative determination of illicit heroin street samples by reversed-phase high-performance liquid chromatography method development by CARTAGO-S. [Pg.9]

Hewitt EF, Lukulay P, Galushko S (2006) Implementation of a rapid and automated high performance liquid chromatography method development strategy for pharmaceutical drug candidates. J Chromatogr A, 1107 79-87. [Pg.160]

RCs were purified using a DEAE chromatography method developed for Rb. capsulatus (10). Bacteria were grown under microaerophilic conditions (nonphotosynthetic) to avoid selection of undesirable revertants. Optical spectra were recorded with a Perkin-Elmer Lambda diode array model 3840 spectrophotometer interfaced to a model 7300 workstation. RC pigment content was determined (6) through the absorbance of near-infrared pigment bands after extraction of RCs with acetone/methanol, 7 2 (voLvol). [Pg.57]

See also Liquid Chromatography Principles Column Technology Mobile Phase Selection. Thin-Layer Chromatography Method Development. [Pg.2571]

Dolan, J.W. Snyder, L.R. Saunders, D.L. Van-Heukelem, L. Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development, n. The use of further changes in conditions. J. Chromatogr. A, 1998, 803 (1-2), 33 50. [Pg.1031]

There is a significant number of ion chromatography methods developed for the determination of metal pollutants in a wide variety of environmental samples. Review of current state of research and progress in ion chromatography as an analytical tool for trace metal analysis in environmental samples is described by Shaw and Haddad.f ... [Pg.1202]

Currently, there is a significant number of ion chromatography methods developed for the determination of inorganic and organic cations in wide variety of sample matrices. Moreover, it has become one of the most widely applied analytical tools for the analysis of complex matrices and for the speciation studies in the field of metal analysis. [Pg.1204]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...