Capacity factor, chromatography

Sherblom, P.M., Eganhouse, R.P. (1988) Correlations between octanol-water partition coefficients and reversed-phase high-performance liquid chromatography capacity factors. J. Chromtogr. 454, 37-50. 

Vowles and Mantoura [38] determined sediment-water partition coefficients and the high-performance liquid chromatography capacity factors for 14 alkylbenzene and polyaromatic hydrocarbons. The partition coefficients correlated well with the alkyl-cyano capacity factors, and it was concluded that this phase gave a better indication of sorption on sediment than either the octanol or octadecylsilane phases. 

Relationships such as Eqs. (45) and (46) have been utilized extensively in correlating solubility properties (such as gas/liquid and liquid/liquid partition coefficients), retention volumes in gas/solid chromatography, capacity factors in high-pressure liquid chromatography, etc.199 200 For instance, gas/liquid partition coefficients for each of 35 different liquid stationary phases were represented with R > 0.985.205 Other applications have been in biochemical and pharmacological areas,199 200 e.g., enzyme inhibition and pollutant effects. 

Perkin Trans. 2, 2087 (1988). Solute-Sovent Interactions in Chemistry and Biology. Part 7. An Analysis of Mobile Phase Effects in High Pressure Liquid Chromatography Capacity Factors and Relationships of the Latter with Octanol-Water Partition Coefficients. 

A convenient point of departure is that of the increasingly popular quantitative structure activity relationships (QSAR) mentioned above [696,699,11], which derive adsorbate-adsorbent interaction indices from, for example, water solubility data, molecular connectivities [697], n-octanol-water partition coefficients, reversed-phase liquid chromatography capacity factors [723], or linear solvation energy relationships (LSER). 

Arunyanart M and Cline Love LJ (1984) Model for micellar effects on liquid chromatography capacity factors and for determination of micelle-solute equilibrium constants. Analytical Chemistry 56 1557-1561. 

Selectivity In chromatography, selectivity is defined as the ratio of the capacity factors for two solutes (equation 12.11). In capillary electrophoresis, the analogous expression for selectivity is... 

Salt Effects. The definition of a capacity factor k in hydrophobic interaction chromatography is analogous to the distribution coefficient, in gel permeation chromatography ... 

The chromatography literature contains a vast amount of dispersion data for all types of chromatography and, in particular, much of the data pertains directly to GC and LC. Unfortunately, almost all the data is unsuitable for validating one particular dispersion equation as opposed to another. There are a number of reasons for this firstly, the necessary supporting data (e.g., diffusivity data for the solutes in the solvents employed as the mobile phase, accurate distribution and/or capacity factor constants (k")) are not available secondly, the accuracy and precision of much of the data are inadequate, largely due to the use of inappropriate apparatus with high extracolumn dispersion. 

A computer program was compiled to work out the ray-tracing of UV detector of high performance capillary electrophoresis at the investigation of 5 and 6 (98MI59). The capacity factor of 5 at different temperature and at different mobile phase compositions was experimentally determined in bonded-phase chromatography with ion suppression (98MI15). 

Solvent optimization in reversed-phase liquid chromatography is commenced by selecting a binary mobile phase of the correct solvent strength to elute the seuaple with an acceptable range of capacity. factor values (1 < k <10 in general or 1 < k < 20 when a larger separation capacity is required). Transfer rules (section 4.6.1) are then used to calculate the composition of other isoeluotropic binary solvents with complementary selectivity. In practice, methanol, acetonitrile and tetrahydrofuran are chosen as the selectivity adjusting solvents blended in different... 

Yamamoto, A., Hayakawa, K., Matsunaga, A., Mizukami, E., and Miyazaki, M., Retention model of multiple eluent ion chromatography. A priori estimations of analyte capacity factor and peak intensity /. Chromatogr., 627,17,1992. 

Razak, J. L. Cutak, B. J. Larive, C. K. Lunte, C. E., Correlation of the capacity factor in vesicular electrokinetic chromatography with the octanokwater partition coefficient for charged and neutral analytes, Pharm. Res. 18, 104—111 (2001). 

Hamisch, M., Mockel, H.J., Schule, G. (1983) Relationship between log Pow shake-flask values and capacity factors derived from reversed-phase high-performance liquid chromatography for n-alkylbenzenes and some OECD reference substances. J. Chromatogr. 282, 315-332. 

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