High performance liquid chromatography capacity factor

Sherblom, P.M., Eganhouse, R.P. (1988) Correlations between octanol-water partition coefficients and reversed-phase high-performance liquid chromatography capacity factors. J. Chromtogr. 454, 37-50. 

Vowles and Mantoura [38] determined sediment-water partition coefficients and the high-performance liquid chromatography capacity factors for 14 alkylbenzene and polyaromatic hydrocarbons. The partition coefficients correlated well with the alkyl-cyano capacity factors, and it was concluded that this phase gave a better indication of sorption on sediment than either the octanol or octadecylsilane phases. 

Hamisch, M., Mockel, H.J., Schule, G. (1983) Relationship between log Pow shake-flask values and capacity factors derived from reversed-phase high-performance liquid chromatography for n-alkylbenzenes and some OECD reference substances. J. Chromatogr. 282, 315-332. 

DK Lloyd, A Ahmed, F Pastore. A quantitative relationship between capacity factor and selector concentration in capillary electrophoresis and high-performance liquid chromatography evidence from the enantioselective resolution of benzoin using human serum albumin as chiral selector. Electrophoresis 18 958-964, 1997. 

Correlations with Capacity Factors Obtained from High Performance Liquid Chromatography (RP-HPLC)... 

Experimental retention times or capacity factors generated by reverse phase high performance liquid chromatography (RP-HPLC) (Vowles and Mantoura, 1987 Hodson and Williams, 1988 Pussemier et al., 1990 Szabo et al., 1990a,b Hong et al., 1996) also have been correlated with Koc. 

Experimentally determined retention times or capacity factors (k) generated by reverse phase, usually octadecylsilane (ODS), high performance liquid chromatography (RP-HPLC) have been used widely to estimate Kow values (McDuffie, 1981 Haky and Young, 1984 Sarna, 1984 Doucette and Andren, 1988). More recently, this approach has been used to directly estimate Koc (Vowles and Mantoura, 1987 Hodson and Williams, 1988 Szabo et al., 1990 Kordel et al., 1993 Kordel et al., 1995 Hong et al., 1996). This is not strictly an estimation method because it relies on the acquisition of experimental retention times. 

The use of cyclodextrins as the mobile phase components which impart stereoselectivity to reversed phase high performance liquid chromatography (RP-HPLC) systems are surveyed. The exemplary separations of structural and geometrical isomers are presented as well as the resolution of some enantiomeric compounds. A simplified scheme of the separation process occurring in RP-HPLC system modified by cyclodextrin is discussed and equations which relate the capacity factors of solutes to cyclodextrin concentration are given. The results are considered in the light of two phenomena influencing separation processes adsorption of inclusion complexes on stationary phase and complexation of solutes in the bulk mobile phase solution. 

The LFER-based retention parameter in high-performance liquid chromatography (HPLC) is the logarithm of the phase capacity ratio or retention factor k. The capacity... 

Kim, K.H. (1995c). Description of the Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Capacity Factors and Octanol-Water Partition Coefficients of 2-Pyrazine and 2- ridine Analogues Directly from the Three-Dimensional Structures Using Comparative Molecular Field Analysis (CoMFA) Approach. Quant.Struct.-Act.Relat., 14, 8-18. 

Kim, K.H. (1995c) Description of the reversed-phase high-performance liquid chromatography (RP-HPLC) capacity factors and octanol—water partition coefficients of 2-pyrazine and 2-pyridine analogues directly from the three-dimensional structures using comparative molecular field analysis (CoMFA) approach. Quant. Struct. -Act. Rdat., 14, 8-18. 

A number of other surrogate procedures have been developed. These include the use of reverse-phase thin-layer chromatography (TLC) (Bruggeman et al. 1982 Renberg et al. 1985) to measure relative mobilities (Rm) or reverse-phase high-performance liquid chromatography (HPLC) to measure capacity factors (Veith et al. 1979b). These values are then correlated with experimentally established Pow values for standard compounds, and the correlation is then used to calculate values of Pow for the unknown compounds. 

Separation of bile acid-CoA derivatives from unreacted coenzyme A can be effected by high-performance liquid chromatography. With a radial compression C,g column containing 5-/tm particles, coenzyme A was eluted with or immediately following the solvent front with a solvent system of 2-propanol/50 mM phosphate buffer (pH 7.0) (160/340) and a UV-detector set at 254 nm. Capacity factors for the above CoA derivatives are 1.50, 3.50, 4.18 and 9.50. CoA derivatives of comparable 5a-bile acids, purified and separated in a similar manner were eluted just prior to the comparable 5)8-derivative [36]. CoA derivatives of 5jS- and 5a-cholate and de-oxycholate were separable with this solvent system. 

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