Chromatin analysis

Uncultured cells have also been used to a limited extent for fetal sex determination. This Is possible because the cells can be appropriately stained and examined for the presence of sex chromatin, characteristic of female cells, and for the fluorescent Y-body, characteristic of male cells (33,34). However, these cytologic techniques are not completely reliable, and It Is still desirable to determine fetal sex by direct chromosome analysis (35). [Pg.77]

Orlando, V., Strutt, H., and Paro, R. (1997) Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods 11, 205-214. [Pg.1100]

Owen-Hughes T, Workman JL (1994) Experimental analysis of chromatin function in transcription control. Crit Rev Eukaryot Gene Expr 4 403 41... [Pg.27]

Jiang W, Nordeen SK, Kadonaga JT (2000) Transcriptional analysis of chromatin assembled with purified ACF and dNAPl reveals that acetyl-CoA is required for preinitiation complex assembly. J.Biol.Chem 22 39819-39822... [Pg.123]

Antibiotic Mg complex induced alteration in the ultrastructural changes in the native and HI depleted chromatin were monitored by thermal melting analysis, polyacrylamide gel mobility assay, dynamic light scattering experiments and transmission electron microscopic studies. Micrococcal nuclease digestion is the biochemical probe to assess the accessibility of the antibiotic Mg + complexes to nucleosomal DNA. [Pg.157]

Notwithstanding the importance of in-depth analysis of the structural features of the association of the anti-gene transcription inhibitors with DNA, it is clear that structural studies on the association of these compounds with chromatin and its components like nucleosome and HI histone under different conditions are essential... [Pg.159]

Bryan EJ, Jokubaitis VJ, Chamberlain NL, Baxter SW, Dawson E, Choong DY, Campbell IG (2002) Mutation analysis of EP300 in colon, breast and ovarian carcinomas. Int J Cancer 102 137—141 Cairns BR (2001) Emerging roles for chromatin remodeUng in cancer biology. Trends Cell Biol 11 S15-S21... [Pg.254]

Analysis of the chromatin organization of integrated HIV-1 identified a single major nuclease-hypersensitive site in the 8 kb region located between the two LTRs (Verdin, 1991 Van Lint et al, 1994). This hypersensitive site, centered around nt... [Pg.385]

Abbreviation Bp, nucleotide base pairs cDNA, complementary DNA ChIP, chromatin Immunoprecipi-tation Cy5, cyanine 5-dCTP Cy3, cyanine 3-dCTP ESTs, expressed sequence tags FDR, false discovery rate MIAME, minimum information about a microarray experiment mRNA, RNA, messenger NIA, National Institutes of Aging RFUs, relative fluorescence units RT-PCR, reverse transcriptase polymerase chain reaction SAGE, serial analysis of gene expression SAM, significance analysis of microarrays... [Pg.388]

The phenylselenocysteine has also been used successfully to chemically append analogues of methyl- or acetyl-lysine, important histone modifications that can contribute to chromatin structure and accessibility of transcriptional machinery in eukaryotes. By introducing phenylselenocysteine into the Xenopus histone H3, both acetyl-lysine and mono-, di-, and trimethyl-lysine analogues were appended to the purified unnatural amino acid-containing FI 3 protein (Figure 10). " Additionally, the H3 protein with a modification mimicking acetylation of lysine 9 can be deactylated by a histone deacetylation complex and is also a substrate for phosphorylation by Aurora B kinase. Such purified and chemically labeled histones are likely functional in nucleosomes, and preparation of specifically modified histones for comprehensive analysis of chromatin structure and accessibility is particularly suited to this chemical labeling technique. [Pg.605]

DNA IN CHROMATIN FROM GENOME-WIDE SEQUENCE ANALYSIS TO THE MODELING OF REPLICATION IN MAMMALS... [Pg.203]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...