Chromatin data analysis

Finally, targeted active demethylation has been put forward as an explanation for the unmethylated state of CpG islands (Fig. 3e). In this model, features of active chromatin structure (e.g., histone hyperacetylation) cause active demethylation of associated sequences [43,44]. Based on a broad analysis of existing data, Szyf... 

Furthermore, the acceptor proteins were analyzed by acid urea polyacrylamide gel electrophoresis (Fig. 2). Histones H2B, HI and protein A24 were found to be poly-(ADP-ribosyl)ated in native chromatin while in histone Hl-depleted chromatin and core particles it was found that histone H2B and protein A24 were poly(ADP-ribosyl)-ated. The presence of the hyper(ADP-ribosyl)ated forms of histone H2B on each of these various levels of chromatin structure have been confirmed by western blot analysis and by two-dimensional polyacrylamide gel electrophoresis (data not shown). The poly(ADP-ribosyl)ation of protein A24 have also been demonstrated by this last technique. 

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