Chinese hamster ovary cells interferons produced

After this period, there was an accelerated use of animal cells. Namalwa cells (Finter et al., 1991) were used to produce alpha-interferon by Wellcome in 1986. Vero cells (a cell line derived from monkey) were used for anti-rabies vaccine. Hybridomas were considered acceptable for mAb production and the use of genetically modified CHO (Chinese hamster ovary) cells was authorized for the production of tissue plasminogen activator (tPA) by Genentech in 1986. 

Flayter PM, Curling EMA, Baines AJ., Jenkins N, Salmon I, Strange PG, Tong JM, Bull AT (1992), Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human interferon-. Biotechnol. Bioeng. 39 327-335. 

Since calorimetric data are a measure of rate, it is possible to link heat output with the metabolic activity of human (and animal) cells. Both types of cell have been extensively studied by flow calorimetry one example is the study and optimization of Chinese hamster ovary cells producing recombinant interferon y-... 

Interferon-/ -la (Avonex and Rebif) is a natural-sequence glycosolated interferon produced in recombinant Chinese hamster ovary cells. Avonex is given as 30 meg (6 million lU) intramuscularly (IM) once weekly. The prefllled syringes should be refrigerated but may be kept at room temperature for 30 days. Avonex costs approximately 12,915 per year. Rebif is given as 44 meg subcutaneously three times weekly. It is supplied in a 0.5-mL prefilled syringe with an... 

A. General description Interferon beta-la is produced by Chinese hamster ovary (CHO) cells into which the human interferon beta gene has been introduced. The amino-acid sequence of the recombinant protein produced by these cells is identical to naturally occurring interferon beta. Interferon beta-la is a single-chain glycosylated polypeptide, 166 amino-acid residues in length, with an approximate molecular weight of 22.5 kDa. 

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