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Chemicals DEAE cellulose

James, K. and Stanworth, D. R. (1964) Studies on the chromatography of human serum proteins on diethylaminoethyl(DEAE)-cellulose. (1) The effect of the chemical and physical nature of the exchanger. J. Chromatog. 15, 324-335. [Pg.22]

Chemically modified cellulose supports are also used in TLC, either as fibres or a crystalline powder. The most widely known of these is DEAE-cellulose, a basic phase containing diethylaminoethyl groups. The hydrophilic character of other polar phases with ion exchange properties can be used for the separation of ampholytes. [Pg.88]

The extracellular polysaccharides of Rhizobium meliloti 201 have been examined by using enzymic degradation and chemical procedures.314 A mixture of polysaccharides produced by the bacterium, when incubated with a bacterial enzyme that hydrolyzed one of these, gave oligosaccharides that could be separated by DEAE-cellulose chromatography. The major fraction was a pentasaccharide, for which methylation analysis and Smith... [Pg.228]

Several functional groups have been used to obtain cellulose anion exchangers [aminoethyl (AE), diethyl-aminoethyl (DEAE)], or cation exchangers [car-boxymethyl (CM), phosphate (P)] for thin-layer chromatography. PEI cellulose is not a chemically modified cellulose, but a complex of cellulose with polyethyleneimine. These cellulose exchangers are particularly useful for the separation of proteins, aminoacids, enzymes, nucleobases, nucleosides, nucleotides, and nucleic acids. [Pg.1639]

Materials. The following were obtained from commercial sources Swims medium S77, horse serum, and fetal calf serum (Grand Island Biologicals) MTX (Lederle Laboratories) was purified by DEAE cellulose prior to use (11) crystalline BSA, poly-L-lysine (M 35,000), hypoxanthine and thymidine (Sigma Chemical Co.) 1-etnyl-3-(3 -dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Story Chemicals) folinic acid (ICN Pharmaceuticals) Blue Dextran (Pharmacia). The concentrations of the folate derivatives and MTX were determined by their respective extinction coefficients (13). MTX-BSA was synthesized according to a previously described procedure ( ) in which MTX is coupled via a terminal carboxyl group to -amino groups contained in the albumin molecule. [Pg.256]

Interestingly, this enzyme also accepted cis- and tra s-2,3-disubstituted olefins. Moreover, it appeared that racemic czs-2,3-epoxyheptane led to almost total deracemisation, thus affording the corresponding R,R) diol with 79% chemical yield and 91 % ee [187] (Fig. 15). This is due to the fact that the regioselectivity of the enzymatic attack on each of the two enantiomers was different. Recent work described that this enzyme could be immobilized on DEAE-cellulose, thus... [Pg.178]

In the case of acidic glycolipids the relative proton affinity of chemicals can shift the balance for negative ionisation in favor of co-eluted compounds. Pre-analytical separation under acidic conditions serves also to reduce as much as possible the dispersion in the MS spectrum of the metabolite into multiple m/ z representing the various adducts of counterions Na, K, NH4, organic amines, ... which improves sensitivity of the test. Sulfatides are lost during the partition between the hexane and the methanol/water phase. The analysis of sulfatides involves the isolation of the glycosphingolipid fraction and the subsequent separation of sulfatides from neutral lipids by chromatography on DEAE-sephadex or DEAE-cellulose column (the variety of methods are referenced in the website CyberLipid (http //www.cyberlipid.org/). [Pg.582]

FIGURE 1 Elution profile of calcium binding proteins from a DEAE-cellulose column. Solvent system used was 20 mM imidazole (pH 6.2), 1 mM EDTA, containing 1000 ml of a linear NaCl gradient from 0 to 0.25 M. Reprinted with permission from Biochemistry (29). Copyright 1990 American Chemical Society. [Pg.106]

All reagents and chemicals were obtained from Sigma Chemical Company. DEAE-cellulose for column chromatography was purchased from Whatman. [Pg.1244]

C]Thymidine 5 -monophosphate and [6- H]thymidine were purchased from the Radiochemical Centre, Amersham. DE-81 (DEAE cellulose paper) disks, 2.3 cm diameter, were obtained from Whatman Biochemical Ltd., Maidstone. All other chemicals used were of analytical grade purchased from either BDH or Sigma, London. [Pg.554]

Chromatography matrices. DEAE-cellulose (DE-52, Whatman) hydroxylapatite (Bio-gel HTP, Bio-Rad), Mono Q anion exchange columns (Pharmacia Fine Chemicals) and Ni-NTA nickel affinity chromatography support (Qiagen)... [Pg.93]

Preparation of urine samples for chemical assay was as follows. Kaolin and acetone treated powder (see below) was dissolved in 0.2M sodium phosphate buffer, pH 5.8, and subjected to successive chromatographic separations on Amberlite CG-50 and DEAE-cellulose columns employing conditions identical with those for pituitary samples. The urine fractions isolated following CG-50 and DEAE-cellulose chromatography were designated as fractions UH and UL respectively. [Pg.441]


See other pages where Chemicals DEAE cellulose is mentioned: [Pg.221]    [Pg.236]    [Pg.112]    [Pg.200]    [Pg.170]    [Pg.171]    [Pg.80]    [Pg.1404]    [Pg.233]    [Pg.310]    [Pg.328]    [Pg.328]    [Pg.165]    [Pg.213]    [Pg.212]    [Pg.607]    [Pg.212]    [Pg.232]    [Pg.60]    [Pg.774]    [Pg.777]    [Pg.189]    [Pg.258]    [Pg.68]    [Pg.935]    [Pg.731]    [Pg.798]    [Pg.798]    [Pg.66]    [Pg.220]    [Pg.263]    [Pg.31]   
See also in sourсe #XX -- [ Pg.175 ]




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