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Chromatography matrices

The most commonly used chromatography matrices are affinity, ion-exchange, reverse-phase, and molecular sieving matrices. An affinity matrix contains a specific ligand that binds only the protein of interesta specific enzyme inhibitor or a particular DNA sequence can be used this way. The protein of interest binds to the column and the other proteins in the mixture pass through. The protein is then eluted with a solvent that breaks the interaction between the ligand and the protein so that the protein can be eluted in pure form. [Pg.119]

Recent references to the preparation of active immobilized forms of enzymes (for use as immobilized enzymes) and immunologically active macromolecules (for use as immunoadsorbents) and of various afflnants (for use as affinity-chromatography matrices) using agarose cyclic imidocarbonate are summarized in the Tables that follow (pp. 451-457), the separate categories being the subject of... [Pg.450]

Assessment of the relative suitabilities of agarose cyclic imidocarbonate and other agarose derivatives as affinity-chromatography matrices... [Pg.453]

Albumin activated with glutaraldehyde has been coupled with catalase and the product used in a thermometric enzyme-linked immunosorbent assay. Folic acid has been used to modify albumin in a carbodi-imide-mediated reaction. The folate-derivative was then coupled to agarose and used for affinity chromatography. Albumin alkylated with l-[ C]iodoacetamide has been used in the development of a new method for preparing affinity chromatography matrices. ... [Pg.554]

Chromatography matrices. DEAE-cellulose (DE-52, Whatman) hydroxylapatite (Bio-gel HTP, Bio-Rad), Mono Q anion exchange columns (Pharmacia Fine Chemicals) and Ni-NTA nickel affinity chromatography support (Qiagen)... [Pg.93]

Cross-linking of desulphated agarose with epichlorohydrin gave a derivative that exhibited improved properties for the preparation of affinity-chromatography matrices. ... [Pg.454]

See other pages where Chromatography matrices is mentioned: [Pg.774]    [Pg.270]    [Pg.28]    [Pg.512]    [Pg.164]    [Pg.242]    [Pg.394]    [Pg.443]    [Pg.106]    [Pg.422]    [Pg.458]    [Pg.549]    [Pg.628]    [Pg.38]    [Pg.1665]    [Pg.713]    [Pg.306]    [Pg.358]    [Pg.388]    [Pg.388]    [Pg.222]    [Pg.191]    [Pg.83]    [Pg.296]    [Pg.611]    [Pg.590]    [Pg.335]    [Pg.464]    [Pg.479]    [Pg.154]    [Pg.527]    [Pg.537]    [Pg.20]    [Pg.342]    [Pg.416]    [Pg.424]    [Pg.433]    [Pg.450]    [Pg.450]    [Pg.450]    [Pg.454]   
See also in sourсe #XX -- [ Pg.388 , Pg.389 , Pg.390 , Pg.391 , Pg.392 , Pg.393 , Pg.394 , Pg.395 , Pg.396 , Pg.397 , Pg.398 ]



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