Chemical industry, high-performance liquid chromatography

Methanol, n-hexane, ethyl acetate, distilled water, sodium chloride, sodium sulfate, reagent grade for residue analysis (Wako Pure Chemical Industries, Ltd, Japan) Acetonitrile, methanol, distilled water, reagent grade for high-performance liquid chromatography... 

Hou W, Watters JW, McLeod HL (2004) Simple and rapid docetaxel assay in human plasma by protein precipitation and high-performance liquid chromatography-tandem mass spectrometry. Journal of Chromatography B 804 263-267 Schuhmacher J, Zimmer D, Tesche F, Pickard V (2003) Matrix effects during analysis of plasma samples by electrospray and atmospheric pressure chemical ionization mass spectrometry practical approaches to their elimination. Rapid Communications in Mass Spectrometry 17 1950-1957 Shah PW (2001) Guidance for Industry Bioanalytical Method Validation U.S. Department of Health and Human Services, Food and Drug Administration... 

Materials. Narrow PS standards were obtained from Pressure Chemical Company (Pittsburgh, PA). A broad PS standard (MW = 250,000) was obtained from American Polymer Standards Corp. (Mentor, OH). The PMMA materials were Perspex CQ UV obtained from Imperial Chemical Industries (Wilmington, DE) and UV 52E obtained from Pharmacia Ophthalmics Inc.(Monrovia, CA). High-performance liquid chromatography-grade THF from JT Baker Inc. (Phillipsburg, NJ) was used as a carrier for all ThFFF and SEC experiments. The polymer solutions had concentrations of 0.2% (wt/vol). 

A variety of MS formats are widely accepted and applied in the pharmaceutical industry. The specific MS application is often defined by the sample introduction technique. The pharmaceutical applications highlighted in this article feature two types of sample introduction techniques dynamic and static. Dynamic sample introduction involves the use of high-performance liquid chromatography (HPLC) on-line with MS. The resulting liquid chromatography/mass spectrometry (LC/MS) format provides unique and enabling capabilities for pharmaceutical analysis. The electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) modes are the most widely used. Static sample introduction techniques primarily use matrix-assisted laser desorption/ionization (MALDI). ... 

Many of the most important chemical questions in the pharmaceutical industry involve the analysis of complex mixtures. Identification of low-level metabolites and drug substance impurities usually requires high-performance liquid chromatography for the separation of these mixtures or isolation of a compound of interest from a sample matrix. In these analyses, the structural information obtainable for the low-level compounds is limited by the type of detection used. The coupling of HPLC and mass spectrometry has become routine and provides useful molecular weight and fragmentation information, but this is often not enough for complete structure elucidation. 

The technology of high performance liquid chromatography has been successfully extended from the analytical scale to the process scale. The ability to control the various operation parameters to scale up directly from the laboratory to the pilot plant and beyond to the production environment has been developed. This technology can be combined with other separations technologies, such as membrane separations, to provide particle-free solvents, ultrapure products, and concentrated product streams. This will give the opportunity to deal with future separations problems of the chemical process industry. 

A recently published article did not confirm the neurotoxic effect of PrP106-126 and indicated that the possible source of the neurotoxic activity could be an high-performance liquid chromatography (HPLC) contaminant (Kunz et al, 1999). In this regard, it is noteworthy that the neurotoxicity of PrP106-126 has been successfully replicated in many laboratories throughout the world. Furthermore, PrP 106-126 was obtained from several different sources, both industrial and academic, and the possibility that a chemical contaminant with similar properties was systematically and exclusively associated with PrP106-126 is unrealistic (Forloni et al, 2000). 

Lisa M, Holcapek M. Triacylglycerols profiling in plant oils important in food industry, dietetics and cosmetics using high-performance liquid chromatography—atmospheric pressure chemical ionization mass spectrometry. J Chromatogr A 2008 1198—1199 115-30. 

---