Cheese lactose fermentation

Cheese whey soHds contain 70—75% lactose, which can serve as the carbon source for lactose fermenting yeasts such as Klujveromjcesfragilis. The total volume produced is considerably smaller than for the other yeasts described. 

Fermentation of lactose during the growth of micro-organisms in milk has a major effect on its redox potential. The decrease in the h of milk caused by the growth of lactic acid bacteria is shown in Figure 11.3. A rapid decrease in h occurs after the available 02 has been consumed by the bacteria. Therefore, the redox potential of cheese and fermented milk products is negative. Reduction of redox indicators (e.g. resazurin or... 

Excessive or insufficient acid development during manufacture can produce variability in the moisture content of cheese and defects in flavor, body, texture, color, and finish (Van Slyke and Price 1952). The rate of lactose fermentation varies with the type of cheese, but the conversion to lactic acid is virtually complete during the first weeks of aging (Van Slyke and Price 1952 Turner and Thomas 1980). Very small amounts of lactose and galactose may be found in cheese months after manufacture. (Huffman and Kristoffersen 1984 Turner and Thomas 1980 Harvey et al. 1981 Thomas and Pearce 1981). Turner and Thomas (1980) showed that the fermentation of residual lactose in Cheddar cheese is affected by the storage temperature, the salt level in the cheese and the salt tolerance of the starter used. 

Thomas. T.D., Pearce, K.N. 1981. Influence of salt on lactose fermentation and proteolysis in Cheddar cheese. N.Z. J. Dairy Sci. Technol. 16, 253-259. 

Keywords Succinic acid Cheese whey Lactose Fermentation ... 

Abstract The processes of lactic acid production include two key stages, which are (a) fermentation and (h) product recovery. In this study, fiee cell of Bifidobacterium longum was used to produce lactic acid from cheese whey. The produced lactic acid was then separated and purified from the fermentation broth using combination of nanofiltration and reverse osmosis membranes. Nanofiltration membrane with a molecular weight cutofif of 100-400 Da was used to separate lactic acid from lactose and cells in the cheese whey fermentation broth in the first step. The obtained permeate from the above nanofiltration is mainly composed of lactic acid and water, which was then concentrated with a reverse osmosis membrane in the second step. Among the tested nanofiltration membranes, HL membrane from GE Osmonics has the highest lactose retention (97 1%). In the reverse osmosis process, the ADF membrane could retain 100% of lactic acid to obtain permeate with water only. The effect of membrane and pressure on permeate flux and retention of lactose/lactic acid was also reported in this paper. 

Combined nanofiltration and reverse osmosis membranes could successfully separate and concentrate lactic acid from cheese whey fermentation broth. Nanofiltration membrane could retain about 97% of lactose to obtain permeate mainly containing lactic acid and water. The highest lactose retention of 97% was obtained with the HL membrane. The tested reverse osmosis membranes successfully separated lactic acid from water. Nearly 100% of lactic acid retention was obtained with the ADF membrane. 

Lactate. Hard rennet cheeses represent a selective habitat for propionic acid bacteria, since they contain lactate formed as the end product of lactose fermentation by lactic acid bacteria. Unlike many other bacteria, propioni-bacteria can utilize lactate efficiently, which is the reason why propioni-bacteria are so abundant in hard, ripened cheeses. Propionic acid bacteria use lactate best in the presence of yeast extract (Antila, 1954), but even higher stimulatory effect is exerted by cell-free extracts of lactic acid bacteria. Streptococcus thermophilus and Lactobacillus spp. (Hietaranta and Antila, 1953). Lactate as a carbon source supports higher growth rates of propionic acid bacteria than lactose (El-Hagarawy et al., 1954). 

The main role of propionic acid bacteria in cheese ripening consists in the utilization of lactate produced by lactic acid bacteria as an end product of lactose fermentation. Lactate is then transformed into propionic and acetic acids and CO2. The volatile acids provide a specific sharp taste and help preserve a milk protein, casein. Hydrolysis of lipids with the formation of fatty acids is essential for the taste qualities of cheese. The release of proline and other amino acids and such volatile compounds as acetoin, diacetyl, dimethylsulfide, acetaldehyde is important for the formation of cheese aroma. Carbon dioxide released in the processes of propionic acid fermentation and decarboxylation of amino acids (mainly) forms eyes, or holes. Propionic acid bacteria also produce vitamins, first of all, vitamin At the same time, an important condition is to keep propionibacteria from growing and producing CO2 at low temperatures, since this would cause cracks and fissures in cheese. 

If lactose-fermenting P. shermanii is used, cheese whey can be used as a substrate. From the whey containing 12% of dry substances, only 50% of lactose is utilized by P. shermanii, producing 1.6-2.2% solution of propionic acid (Bodie et al., 1987). In the combined batch culture, composed of P. shermanii and Lactobacillus casei, lactose is completely utilized and a 3% solution of propionic acid is produced in 52 h. When the medium is partially replaced during cultivation, a 4.5% solution of propionic acid is obtained by raising the concentration of dry substances in the whey up to 18% the production is increased up to 6.5% in mixed culture. For industrial production it is advisable to add a reducing agent to the medium (see above. Chapter 3), then the ratio of propionic to acetic acid will be increased (Emde and Schink, 1990). 

Lactose is mainly used as a fermentation substrate for lactic acid bacteria in dairy products, such as yogurt and cheese. These bacteria break down lactose into lactic acid, which solidifies the milk, and creates an acid environment that favors the benign lactic acid bacteria over those that are more harmful. 

Whey powders, demineralized whey powders, whey protein concentrates, whey protein isolates, individual whey proteins, whey protein hydrolysates, neutraceuticals Lactose and lactose derivatives Fresh cheeses and cheese-based products Functional applications, e.g. coffee creamers, meat extenders nutritional applications Whey powders, demineralized whey powders, whey protein concentrates, whey protein isolates, individual whey proteins, whey protein hydrolysates, neutraceuticals Various fermented milk products, e.g. yoghurt, buttermilk, acidophilus milk, bioyoghurt... 

Acid production is a key feature in the manufacture of all cheese varieties -the pH decreases to about 5 ( 0.3, depending on variety) within 5-20h, at a rate depending on the variety (Figure 10.11). Acidification is normally achieved via the bacterial fermentation of lactose to lactic acid, although an acidogen, usually gluconic acid-<5-lactone, alone or in combination with acid, may be used in some cases, e.g. Mozzarella. 

On acidification to pH 4.6, the caseins coagulate, which is the principle used to manufacture of a family of cheeses which represent about 25% of total cheese consumption and are the principal cheeses in some countries (Appendix 10B). Acidification is traditionally and usually achieved by in situ fermentation of lactose by a Lactococcus starter but direct acidification by acid or acidogen (gluconic acid-d-lactone) is also practised. The principal... 

Raw milk is a unique agricultural commodity. It contains emulsified globular lipids and colloidally dispersed proteins that may be easily modified, concentrated, or separated in relatively pure form from lactose and various salts that are in true solution. With these physical-chemical properties, an array of milk products and dairy-derived functional food ingredients has been developed and manufactured. Some, like cheese, butter, and certain fermented dairy foods, were developed in antiquity. Other dairy foods, like nonfat dry milk, ice cream, casein, and whey derivatives, are relatively recent products of science and technology. This chapter describes and explains the composition of traditional milk products, as well as that of some of the more recently developed or modified milk products designed to be competitive in the modern food industry. 

The physical and chemical characteristics of cheese curd depend on the method used to form the curd matrix. The curd is formed in basically one of two ways acid or enzymatic coagulation. In acid curd cheeses (cottage, baker s, cream), the curd is formed by direct addition of acid to the milk or by lactic acid produced by the fermentation of lactose. As the pH of the milk approaches the isoelectric point of casein (pH... 

The most important fermentative reaction used in dairy processing is the homofermentative conversion of lactose to lactic acid. The efficient manufacture of high-quality cultured products, including most cheese varieties, yogurt, and cultured buttermilk, requires a rapid and consistent rate of lactic acid production. Lactic acid helps to preserve, contributes to the flavor, and modifies the texture of these products. Nearly all starter cultures used to produce acidified dairy products contain one or more strains of lactic streptococci, because these organisms can produce the desired acidity without causing detrimental changes in flavor or texture. Strains of lactic streptococci can be classified as... 

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