Charge-coupled device , image analysis

Recently, an automatic color video image analysis system was developed to quantify antigen expression (androgen receptor) (Kim et al.,T999a). This system provides a linear relationship between the antigen content and mean optical density of the immunoperoxidase-substrate reaction product. Titration of antibody, concentration, and reaction duration of the substrate can be optimized with this system. The imaging hardware consists of a Zeiss microscope, a three-chip charge-coupled-device camera, a camera control board, and a Pentium-based personal computer. 

A fundus camera was modified to provide video angiograms and to deliver one laser beam used to release the content of the liposomes and another laser beam to activate the photosensitizer. The first laser was also used to illuminate the fundus. The output of the charge-coupled device (CCD) camera was fed into a video image enhancer and recorded on magnetic tapes with a high-frequency video recorder. Later, the tape was played back, and video sequences were digitized with a frame grabber for subsequent analysis. 

Reaction product imaging. In this technique, product ions are accelerated by an electric field toward a phosphorescent screen and the light emitted from the screen is imaged by a charge-coupled device. The significance of this experiment to the study of chemical reactions is that it allows for a detailed analysis of the angular distribution of products. 

Chouhan, R.S., VivekBabu, K., Kumar, M.A., Neeta, N.S., Thakur, M.S., Amitha Rani, B.E., Pasha, A., Karanth, N.G., and Karanth, N.G.K., 2006. Detection of methyl parathion using immuno-chemiluminescence based image analysis using charge coupled device. Biosensor and Bioelectronics. 21 1264-1271. 

Garcia Sanchez, F., Navas Diaz, A., and Fernandez Correa, M. R. (1993). Image analysis of photochemically derivatized and charge-couple-device-detected phenothiazines separated by thin layer chromatography. J. Chromatogr. 655 31-38. 

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