Fraction combination

All samples were digested with trypsin and analyzed by cIEF in the first dimension followed by LC-MS/MS as described above. Samples were analyzed in duplicate. Sequence searching was performed using OMSSA. Analysis of the soluble fraction yielded a total of 2856 identified proteins, while the insoluble fraction yielded 3227 proteins. Combined, the fresh-frozen sample yielded 3902 protein identifications. The FFPE portion yielded 2845 protein identifications from 14,178 distinct tryptic peptide sequences, on a par with the fresh-frozen soluble fraction. Combining all identifications gave 4145 proteins. While, the soluble fraction and the FFPE extraction yielded similar numbers of protein identification, both found 25% of their respective protein set uniquely (Fig. 20.4). 

Houtman, C. J., Booij, R, Jover, E., Pascual del Rio, D., Swart, K., Velzen, M. van, Vreuls, R., Legler, J., Brouwer, A. and Lamoree, M.H. (2006). Estrogenic and dioxin-like compounds in sediment fi om Zierikzee harbour identified with CALUX assay-directed fractionation combined with one and two dimensional chromatography analyses. Chemosphere, 65, 2244-2252. 

Large-format gel, multi-fractionation, combined narrow range pi gels... 

J. Wieling, H. Dijkstra, C.K. Mensink, J.H.G. Jonkman, P.M.J. Coenegracht, C.A.A. Duineveld and D.A. Doombos, Chemometrics in Bioanalytical Sample Preparation a Fractionated Combined Mixture and Factorial Design for the Modelling of the Recovery of Five Tricyclic Amines from Plasma after Liquid-Liquid Extraction, Journal of Chromatography, 629(2) (1993) 181-199. 

The mass-action relationship in Eq. 8.40 for the product of the cation and anion vacancy site fractions combined with Eq. 8.49 yields... 

Shkinev, VM., Fedorova, O.M., Spivakov, B.Ya., Mattusch, J., Wennrich, R. and Lohse, M. (1996) Speciation of metals associated with natural water components by on-line membrane fractionation combined with inductively coupled plasma atomic emission and mass spectrometries. Anal. Chim. Acta, 327, 167-174. 

Lattice imaging studies by TEM on a "young" fraction showed extensive regions of crystallinity with minimal evidence of crystallite fracturing. By contrast, similar investigations of an "old" fraction, combined with in situ compositional analysis, revealed small "islands" of crystallinity within a "sea" of disordered material that was once crystalline. Fracture lines at crystallite boundaries are absent. Instead, the small USY crystallites within the "old" fraction are in intimate mixture with the collapsed zeolite. 

The behavioural assay of fractions eluted from silicagel with, respectively, hexane, 10% ether in hexane, 50% ether in hexane and ether, indicated that the activity was confined to the fraction containing Cu acetates (the 10% ether fraction). Combination with other fractions did not increase the activity. 

Schimpf ME (1995) Determination of molecular weight and composition in copolymers using thermal field-flow fractionation combined with viscometry. In Provder T, Barth HG, Urban MW (eds) Chromatographic characterization of polymers hyphenated and multidimensional techniques. American Chemical Society, Washington, DC, pp 183-196... 

The time to ignition remains unchanged for the PE-ZCHS-5 but is reduced for PE-ZCHS-10. The THR is observed to be reduced by approximately 13%, a smaller change than observed when copper-containing additives catalyze cross-linking and thus enhance char formation (5). The amount of smoke measured from cone calorimetry as ASEA increases with the additive fraction. Combining the TGA and cone data, the 5% loading provides the best combination of thermal stability and fire properties. 

Lake Fork, outcrop (244 g) heptane-soluble fraction benzene-soluble fraction combined heptane- and benzene-soluble fractions... 

Determination of Molecular Weight and Composition in Copolymers Using Thermal Field-Flow Fractionation Combined with Viscometry... 

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