Cellular morphology

Apoptotic executioner caspases (caspase-3, -6, -7) constitute a subgroup of the caspase family. These proteases are the workhorses of the apoptotic process as they are responsible for cleaving many down-stream substrates important for cellular morphology, organelle homeostasis, cell cycle arrest, and regulation of transcription and translation. 

HSner, B., Citi, S., Kendrick-Jones, J., Jockusch, B.M. (1988). Modulation of cellular morphology and locomotory activity by antibodies against myosin. J. Cell Biol. 107, 2181-2189. 

Visual imaging of cell population in vitro) using low signal-to-noise ratio phase contrast microscopy can enable systematic monitoring measurements of cell quality, development and apoptosis. In the present study, microscopic evaluations as seen in Fig. 11 did not reveal any significant alteration in cellular morphology upto 1000 ng/ml. 

PI. 2.1 A Cellular morphology of ncuroepithelium. Microvilli multiple array at apex of dendrite from an isolated Frog VNOR cell, scale bar = 1 pm SEM (from Trotier el aL, 1994). 

Fig. 2.14 Cellular morphology of neuroepithelium chemosensory groove in lamellae of the African lungfish Protopterus annectens (Dipnoan) basal, receptor and supporting cells (CB, CRe and CS) MB basement membrane. Owen, x400 (from Derivot, 1984).

Experimental Materials. All the data to be presented for these illustrations was obtained from a series of polyurethane foam samples. It is not relevant for this presentation to go into too much detail regarding the exact nature of the samples. It is merely sufficient to state they were from six different formulations, prepared and physically tested for us at an industrial laboratory. After which, our laboratory compiled extensive morphological datu on these materials. The major variable in the composition of this series of foam saaqples is the aaK>unt of water added to the stoichiometric mixture. The reaction of the isocyanate with water is critical in determining the final physical properties of the bulk sample) properties that correlate with the characteristic cellular morphology. The concentration of the tin catalyst was an additional variable in the formulation, the effect of which was to influence the polymerization reaction rate. Representative data from portions of this study will illustrate our experiences of incorporating a computer with the operation of the optical microscope. 

Fig. 13.2 Cellular morphology of Lactobacillus (L. delbrueckii subsp. bulgaricus and L. casei), Streptococcus thermophilus and Bifidobacterium sp.

At Leica Biosystems Newcastle Ltd., all control cell lines undergo strict quality control evaluation using haematoxylin eosin (H E) and Oracle HER2 Bond IHC System (Leica Microsystems Newcastle, UK) stained sections. This allows for evaluation of the three main cell line characteristics cellular morphology, IHC profile, and core density (see Table 6.1)... 

TABLE 6.1 IHC Profile, Cellular Morphology, and Core Density Quality Control Procedures employed at Leica Microsystems... 

IHC Profile Score Cellular Morphology Result Core Result... 

HSV in human fibroblasts was detected using biotin-labeled HSV DNA probes, streptavidin-HRP complex, and enhanced CL substrate reagent for HRP [56], The presence of HSV DNA was observed in cells infected with clinical samples known to contain the HSV virus fixed at 48 h postinfection, with a sharp topographical localization and a good preservation of cellular morphology. 

Proteins of the cytoskeleton play a central role in the creation and maintenance of cell shapes in all tissues. They serve multiple roles in eukaryotic cells. First, they provide structural organization for the cell interior, helping to establish metabolic compartments. Second, cytoskeletal structures serve as tracks for intracellular transport, which creates and maintains differentiated cellular functions. Finally, the cytoskeleton comprises the core framework of cellular morphologies. 

The maximum non-toxic concentration (MNTC) of each sample was determined according to the method described elsewhere based on cellular morphologic alteration [12], Several concentrations of each sample were placed in contact with confluent cell monolayer and incubated in 5% CO at 37°C for 48 h. MNTC values were determined by comparing treated and controlling untreated cultures. 

Rubber Chemistry and Technology 65,No.5,Nov./Dec.l992,p.932-55 INFLUENCE OF COMPOSITION AND PROCESSING HISTORY ON THE CELLULAR MORPHOLOGY OF THE FOAMED OI.EFINIC THERMOPLASTIC ELASTOMERS Dutta A Cakmak M Akron,University... 

Thermoplastic structural foams with bulk densities not less than 50% of the solid resin densities are considered. Cellular morphology, uniform-density cell behaviour, the I-beam concept in designing, core-density profile and the role of the skin, mechanical properties, and ductile-brittle transitions are discussed. 63 refs. 

Jones R, Carpenter E, Lamprecht Ret al (2009) Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning. PNAS 106 1826-1831... 

The main advantage of evaluating the cytological preparation under the microscope is that it allows better assessment of cellular morphology in oligocellular CSF specimens. A limitation of immunotyping is the relatively high cost of the... 

Many biochemical methods have been developed for the assessment of apoptosis. They are based on the analysis of cellular morphology, DNA fragmentation, or... 

One form of asymmetric division caused by unfavourable conditions is spoliation, which is a highly coordinated process ultimately producing spores which are resistant to adverse environmental conditions. When conditions are favourable, out-growth to vegetative cells then occurs. Streptomycetes and the blue-green bacteria are exceptions to this relatively simple cellular morphology and each of these types of bacteria shows various forms of cellular differentiation, both biochemical and morphological 7,8). 

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