Screening imaging-based

Crystals with very strong diffraction may have an imacceptably high number of saturated reflections. As noted above, such crystals are identified automatically during the crystal evaluation process, based on the average number of overloaded reflections on the screening images. For such crystals, the exposure time is reduced. If a further decrease in the illumination of the sample is required, the intensity of the incoming beam is adjusted. 

Ribeiro E, Stafslien SJ, Casse F, Callow JA, Callow ME, Pieper RJ, Daniels JW, Bahr JA, Webster DC (2008) Automated image-based method for laboratory screening of coating libraries for adhesion of algae and bacterial biofilms. J Comb Chem 10 586-594... 

Jones R, Carpenter E, Lamprecht Ret al (2009) Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning. PNAS 106 1826-1831... 

Jones T, Kang 1, Wheeler D et al (2008) CellProfiler analyst data exploration and analysis software for complex image-based screens. BMC Bioinformatics 9 482... 

Danovi D, Falk A, Humphreys P et al (2010) Imaging-based chemical screens using normal and glioma-derived neural stem cells. Biochem Soc Trans 38 1067-1071... 

Here we describe the construction and use of adenoviruses to express lucif-erase- or GFP-fusion proteins for high-throughput cell-based screens. We constructed adenoviruses that express a cyclin Bl-luciferase reporter protein or unmodified luciferase. This enabled us to screen for small molecules that specifically up-regulated levels of the cyclin B 1-luciferase fusion protein without affecting luciferase. A similar approach was used to construct adenoviruses that express cyclin A-GFP or cyclin Bl-GFP fusion proteins. These viruses were used in an imaging-based screen to identify small molecules that specifically upregulated these proteins or altered their subcellular localization. 

Several direct dye staining procedures can measure total protein biomass, nucleic acid content, or selective uptake by organelles such as mitochondria or lysosomes. Many staining methods are appropriate for small numbers of samples or for image-based high content screening but in most cases, the multistep procedures exhibit limitations for HTS. 

Carpenter, A.E. 2007. Image-based chemical screening. Nat. Chem. Biol. 3, 461 —465. 

Informatics for High Content Image-Based Screening.256... 

INFORMATICS FOR HIGH CONTENT IMAGE-BASED SCREENING... 

Bullen, A. 2008. Microscopic imaging techniques for drug discovery. Nat. Rev. Drug Discov. 7, 54-67. Carpenter, A.E. 2007. Image-based chemical screening. Nat. Chem. Biol. 3, 461-465. 

The application of emerging technologies and systems, including image-based high-content screening, RNA interference, and primary cells... 

Chapter 8 Image-Based High-Content Screening.143... 

Jung, S. O., Ro, H. S., Kho, B. H., Shin, Y. B., Kim, M. G., Chung, B. H. (2005). Surface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors. Proteomics, 5, 4427-4431. 

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