Cellular diagnostics

In vitro Diagnosis. Unfortunately, there is no reliable in-vitro allergy diagnostic test available for routine use. Gall et al. [27] had used a self-made radioallergosorbent test with coupled LA to polystyrol discs, however, all the patients were negative. In-vitro cellular diagnostic assays have not been described as useful until now. 

The staining of germinated pollen of Hippeastrum hybridum with colchicine demonstrates green-yellow emission of microtubules (better vision in black-white image) around nuclei of pollen grain (threads at the division of the nucleus) and spermium on the tip of the pollen tube, where spermium moves, as well as in some bridge sites of the tube (Fig. 10). The similar fluorescent allelochemicals may be also used as fluorescent dyes at the cellular diagnostics (Roshchina, 2005 b). 

A diagnostic method using fluorescence labeled DNA probes to detect and quantify the number complementary chromosomal sequences on a cellular resolution. A related technique that also allows assessment of gene amplifications, but without precise quantification of copy numbers is the chromogenic in situ hybridization (CISH). Here, instead of a fluorescent dye an enzyme that can generate a colored precipitate in the tissue samples is coupled to the DNA probe. 

In Chapter 8.5, B. Veyssiere exposes the state of knowledge in detonations. Particular features of the complex multidimensional structure of detonations are presented in relation with the recent results obtained either by nonintrusive optical diagnostics or numerical simulations from high performance calculations. The role of transverse waves in detonation propagation, the existence of correlations between the characteristic dimension of the cellular structure and the critical conditions for detonation initiation and detonation transmission, and the influence of the nonmonotonous heat release process behind the front are examined. Recent developments in the study of spinning detonations are also discussed. 

The book is divided into 3 sections. Section I.Cellular Model Systems, includes 3 chapters (Allelopathy and plant cell diagnostics, Cellular models as biosensors and microalgae for determining the effects of allelochemicals). Section II. New Methods of Microscopy has 5 chapters (Microscopic methods to study morpho-cytological events during the seed germination,... 

The visible fluorescence of intact living cells is called autofluorescence. If the cells treated with special fluorescent dyes, the cellular common fluorescence also includes the additional light emission. Autofluorescence could be used (i) in express-microanalysis of the accumulation of the secondary metabolites in secretory cells without long biochemical procedures (ii) in diagnostics of cellular damage and (iii) in analysis of cellcell interactions (Roshchina, 2003). During the plant development, secretory... 

Gusterson BA, Ross DT, Ffeath VJ, Stein T (2005) Basal cytokeratins and their relationship to the cellular origin and functional classification of breast cancer. Breast Cancer Res 7(4) 143 148 Ffsu FD, Nielsen TO, Alkushi A, Dupuis B, Huntsman D, Liu CL, van de Rijn M, Gilks CB (2002) Tissue microarrays are an effective quality assurance tool for diagnostic immunohistochem istry. Mod Pathol 15 1374 1380... 

Molecular Imaging (166) deals with in vivo characterization and measurement of biological processes at the cellular and molecular level. With Molecular Imaging, early diagnosis of disease will become possible as the detection of altered biochemical processes largely anticipates the anatomical changes that are the basis of current diagnostic modalities. 

The TGAhas also initiated development of two new regulatory frameworks for in nff ro diagnostic devices (IVDs) and human tissues and cellular therapies, which will include ex vivo reagents, to be harmonised with international best practice. It is expected that implementation will occur from early2006 for IVDs and mid-2006 for human tissues and cellular therapies. 

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