Cell hybridization

Goate, A.M., Cooper, D.N., Hall, C., Leung, T.K., Solomon, E., Li, L. (1987). Localization of a human heat shock hsp70 gene sequence to chromosome 6 and detection of two other loci by somatic cell hybrid and RFLP analysis. Hum. Gen. 75, 123-128. 

Use of rodent or human somatic cell hybrids Permits assignment of a gene to one specific chromosome but not to a subregion. 

VI3. Vora, S and Francke, U., Assignment of the human gene for liver-type 6-phosphofructokinase isozyme (PFKL) to chromosome 21 by using somatic cell hybrids and monoclonal anti-L antibody. Proc. Natl. Acad. Sci. U.S.A. 78,3738-3742 (1981). 

Lebaron, P. Catala, P. Fajon, C. Joux, F. Baudart, J. Bernard, L. A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl. Environ. Microbiol. 1997, 63, 3274-3278. 

Integrated Gasification Fuel Cell Hybrid Power Systems Requirements... 

Miller P, Scholin C. Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridization. J Phycol 1998 34 371-382. 

Bennett, K. L., Lalley, P. A., Barth, R. K. and Hastie, N. D. (1982) Mapping the structural genes coding for the major urinary proteins in the mouse combined use of recombinant inbred strains and somatic cell hybrids. Proc. Natl. Acad. Sci. USA 79, 1220-4. 

In July 1998, Toyota said it would try to have a fuel cell automobile ready by 2003, but later this target date was dropped. Toyota believed that there are major cost problems for onboard reformers and saw direct hydrogen as a big technical challenge. Still, it kept working in these areas and its FCHV (fuel cell hybrid vehicle) became the first vehicle in Japan to be certified under the Road Vehicle Act. 

This is consistent with a fuel-cell drive train (the fuel cell, hybrid battery, motor and auxiliaries) manufacturing cost of about 50/kW, and hydrogen-storage cost of 10/kWh, and a retail price mark-up factor of 1.4 (i.e., the retail price is 1.4 times the manufacturing cost). 

Tests of the fuel cell/hybrid system would be performed to assess integration and system operation of an existing 250-kilowatt fuel cell stack with a commercially available microturbine, and... 

Fuel cell hybrid cars are not a new concept. In the early 1970s, K. Kordesch modified a 1961 Austin A-40 two-door, four-passenger sedan to an air-hydrogen fuel cell/battery hybrid car (71). This vehicle used a 6-kW alkaline fuel cell in conjunction with lead acid batteries, and operated on hydrogen carried in compressed gas cylinders mounted on the roof The car was operated on public roads for three years and about 21,000 km. 

Several perspective trends for development of the research concerning domestic animals cells hybridization and obtaining cell systems for biotechnology, human and veterinary medicine can be determined. They are creating hybrids with macrophages, hepatocytes, neurocytes, nucleated and... 

Thus, Xi exemplifies both extremes of the histone code, namely the general deacetylation of all four core histones and the specific retention of H3 dimethylated at lysine 9/27. It also illustrates the great stability of epigenetic signals carried by the histone tails. The general underacetylation of Xi, the undermethylation of H3 lysine 4 and the selective retention of H3 dimethylated at lysine 9/27, are all maintained in human x hamster somatic cell hybrids in which Xi is the only human chromosome and therefore exists in an almost completely foreign (i.e., hamster) cellular environment [73]. 

Hatsu, M. Ohta, J. Takamizawa, K. Monitoring of Bacillus thermodenitrificans OHT-1 in compost by whole cell hybridization. Can. J. Microbiol. 2002, 48, 848-852. 

To obtain MABs, lymphocytes isolated from the spleen of immunized mice (1) are fused with mouse tumor cells (myeloma cells, 2). This is necessary because antibody-secreting lymphocytes in culture have a lifespan of only a few weeks. Fusion of lymphocytes with tumor cells gives rise to cell hybrids, known as hybridomas, which are potentially immortal. 

CityCell - Fuel cell energy in cities. This projects demonstrates five fuel cell-hybrid electric vehicles in Turin, Berlin, Madrid and Paris. 

The two fuel vehicles began operation late in the summer of 2007. Users maintain logs that document vehicle performance, and tests are ongoing to evaluate the operational experience and the refueling station. Other NREL evaluation projects involve transit buses powered by fuel cell hybrids in Oakland, California, Thousand Palms, California, and Hartford, Connecticut. 

The development of hybridoma technology by Milstein and Kohler in 1975 revolutionized the antibody field and radically increased the purity and specificity of antibodies used in the clinic and for diagnostic tests in the laboratory. Hybridomas consist of antibody-forming cells fused to immortal plasmacytoma cells. Hybrid cells that are stable and produce the required antibody can be subcloned for mass culture for antibody production. Large-scale fermentation facilities are now used for this purpose in the pharmaceutical industry. 

As with the metal-oxygen system, hydrogen-metal cells can be considered as closed galvanic/fuel cell hybrids. They make use of the hydrogen... 

Abbott, C., and Povey, S. (1995) Somatic Cell Hybrids, IRL Press, Oxford... 

Cell Fusion Unlike antibody-secreting cells, myeloma cells, malignant tumor cells of the immune system, can be cultured continuously. Kohler and Milstein (1975) developed a method to fuse (hybridize) B-lymphocytes from the mouse spleen with mouse myeloma cells, so that the fused cell, hybrid-myeloma (or hybridoma) cell, can have the characteristic of the both cell lines that is, the production of specific antibodies and the immortality. Since the hybridoma is derived from a single B-lymphocyte, it produces only one kind of antibody, thus a monoclonal antibody. 

De Weerd-Kastelein, E.A., Keijzer, W., Bootsma, D. (1972). Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization. Nature New Biol. 238, 80-83. 

Magistri L., Ferrari M.L., Traverso A., Costamagna P., Massardo A.F. (2004) Transient analysis of solid oxide fuel cell hybrids part C, whole cycle model. In Proceedings of the ASME Turbo Expo, Vienna, Austria, GT2004-53845. 

Liese E., Gemmen R.S., Jabbari F., Brouwer J. (1999) Technical development issues and dynamic modeling of gas turbine and fuel cell hybrid systems. In Proceedings of the ASME Turbo Expo, Indianapolis, IN, 99-GT-360. 

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