Mesenchymal stem cells , cell-based therapies

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Yamada, Y, Nakamura, S., Ito, K., Sugito, T, Yoshimi, R., Nagasaka, T., Ueda, M., 2010. A feasibility of useful cell-based therapy by bone regeneration with deciduous tooth stem cells, dental pulp stem ceUs, or bone-marrow-derived mesenchymal stem cehs for chnical study using tissue engineering technology. Tissue Eng. Part A 16, 1891-1900. 

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Ponticiello, M.S. et al.. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy, in Osiris Therapeutics, John Whey Sons, 2000. 

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Acronyms and Definitions Mesenchymal Stromal Cells Somatic Stem Cell Therapy Good Manufacturing Practice Cell-Based Medicinal Products Human Platelet Lysate... 

Another novel cardiovascular therapy that has recently come into play is the use of stem cell delivery. These approaches have thus far involved the use of gene delivery mechanisms to essentially force embryonic stem cells into cardiomyocyte differentiation with the goal of producing a cardiac pace-making cell (Arruda et al., 2004). Researchers are also developing methods for utilizing seeded adult mesenchymal stem cells as an implanted base to act as a depot for the delivery of localized gene therapies (Arruda et al., 2004). 

Yamada, Y, Fujimoto, A., Ito, A., Yoshuni, R., Ueda, M., 2006a. Cluster analysis and gene expression profiles a cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering ceU therapy. Biomaterials 27, 3766-3781. 

One of the disadvantages of cell injection is that great precision is required for the effective administration of the cells. Furthermore, very low retention rates are generally observed using these techniques. While the reason for this is not completely understood, it is likely that inflammation, nutrient limitations, and damage due to the injection procedure could be responsible. Scaffold-based cell therapy approaches for the treatment of the myocardium could present a possible solution to this problem. A number of recent studies have described using cell-seeded scaffolds instead of cell injections for cell-based therapy of the myocardium cells. Delivery of cardiomyocytes (Zimmermann et al., 2004), mesenchymal stem... 

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