Cell and Tissue Glycoproteins

References to macromolecules present on cell surfaces have been collected together in a separate section (see p. 337). 

A review of the glycoproteins of connective tissues has discussed methods for isolating them from skin, cornea, cartilage, synovial fluid, tendon, cardiovascular tissue, etc Some properties of the principal L-fucosylated glycoprotein released 

Friman, P.-E. Hellstrom, M. Juvani, and H. Riska, Clinica Chim. Acta, 1977, 76, 357. 

Anderson, in International Review of Connective Tissue Research, ed. D. A. Hall and D. S. Jackson, Academic Press, New York and San Francisco, 1976, p. 251. 

Ostrowski, A. K. Bhargava, E. Dziembor, M. Gizler, J. Gryszkiewicz, and E. A. Barnard, Biochim. 

Yosizawa, Clinica Chim. Acta, 1974, 57, 255. 

Glycopeptide fragments, that are not detected in normal cells, have been isolated from cultured skin fibroblasts of patients with one of four lysosomal 

in Reviews of Neuroscience , ed. S. Ehrenpreis and I. J. Kopin, Raven Press, 

Immunological techniques have demonstrated that carcinoembryonic antigen which had been partially degraded in both protein and carbohydrate moieties did not contain the tumour-associated antigen. The antigen (mol. wt. 

Two classes of carbohydrate-protein are thought to be associated with the isolated genome chromatin of HeLaSs cells one consists of glycoproteins (mol. wts. 1 X 10 —1.9 X 10 ) and the other of glycosaminoglycans of much 

Suzuki, S. Suzuki, N. Nakamura, and T. Koizumi, Biochim. Biophys. Acta, 1976, 428, 166. 

The hydrodynamic properties and molecular weights of the F, M, and S glycoproteins present in the perchloric-acid-soluble fraction of the ascitic fluid 

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Mammalian Cell and Tissue Glycoproteins Considerable structural differences in the carbohydrate moieties of glycoproteins present in various soluble and membrane fractions of rat brain have been noted. ... 

Ebrahim AS, Gopalakrishnan R, Murugesan A, et al. 1995. In vivo effect of vitamin E on serum and tissue glycoprotein levels in perchloroethylene induced cytotoxieity. Mol Cell Biol 144 13-18. 

With cells and tissues in suspension culture, the matrix of deposited materials extracellular to the cell wall often stains for lignin and contains tannins and glycoproteins of yet undefined composition (82). This material binds with synthetic growth regulators introduced into the medium. The chemical nature of these extraneous materials is a strong function of the tissue or cell explant source and of the conditions of culture (40). 

Kdn can be detected in free glycans, glycolipids, and glycoproteins in various cells and tissues from animals that express NeuSAc. The expression level is very low in mammals. 

Glycoprotein antigens with ABH and Lewis blood-group specificities, as well as their immunogenic properties, biosynthesis, and occurrence in malignant cells and tissues, have been reviewed. The chemistry of the MN, P, I, J, and Rh antigens was also discussed. A survey of studies on the immunochemistry of water-soluble A, B, H, Le , and Le antigens and their precursors has also appeared. ... 

Sialic acid is an important component of membrane glycoproteins, which has been described in several reviews (e.g. Cook and Stoddart 1973, Hughes 1976, Jeanloz and Codington 1976, Warren 1976, Glick and Flowers 1978, and many others). Analysis of-subcellular membrane fractions has demonstrated the presence of NeuSAc in all fractions of cultured cells and tissue extracts. 

This definition covers an enormous number of materials of different origin, properties, and function. It would include such diverse substances as ovine (sheep) submaxillary mucin (mol. wt I X 10 ) with some 800 disaccharide units per molecule, ovalbumin (mol. wt. 45,000) with a single octosaccharide unit attached, and a host of other substances differing in their amino acid and carbohydrate content and in their molecular sise and shape (Marshall and Neuberger, 1968). Indeed, the definition would include a majority of extracellular proteins since, as Eyiar (1965) has pointed out, the proteins secreted by cells and tissues arc, in general, glycoproteins. 

Degradation of A -linked glycoproteins completely to amino acids and monosaccharides is necessary to avoid the storage diseases mentioned above that have severe consequences for cells and tissues. This catabolism has been determined to occur in two major parts [9, 10] ... 

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