Cell-adhesion peptides

Synthesis of Biodegradable Poiy(lactic acid-co-lysine) Copolymers Linked to Cell Adhesive Peptide Sequences General Procedure 27 ... 

Kato R, Kaga C, Kunimatsu M et al (2006) Peptide array-based interaction assay of solid-bound peptides and anchorage-dependant cells and its effectiveness in cell-adhesive peptide design. J Biosci Bioeng 101(6) 485—495... 

Fig. 9 Reversible cell adhesion by photochemical control of azobenzene SAMs on gold. Interconversion of Z and E configurations (top). Cell adhesive peptide, RGD, is displayed when SAMs adopt the E configuration at 450-490 nm, and cells adhere and grow (bottom left and right). At 340-380 nm, azobenzene converts to the Z conformation, masking RGD, and cell adhesion is prevented (center bottom). Reproduced from [148] with permission. Copyright Wiley, 2009...

Brandley, B. K. and Schnaar, R. L. (1989). Tumor cell haptotaxis on covalently immobilized linear and exponential gradients of a cell adhesion peptide. Dev. Biol. 135, 74-86. 

One of the most prevalent cell adhesion peptides is the tripeptide arginine-glycine-aspartate (RGD) from fibronectin.f This tripeptide has been used extensively to promote adhesion and spreading of many cell types. For example, polyurethane surface can be activated and coupled to RGD via the carboxy terminus. Surfaces containing photo-activable groups, such as benzophenone or aryl azide, can be modified easily with RGD by UV radiation. This tripeptide and other... 

Besides solid material surfaces, functional water-soluble polymers have also been created to promote cell-cell adhesion in aqueous media. For example, cell adhesion peptides of RGD or tyrosine-isoleucine-glycine-serine-arginine (YIGSR) have been attached to the ends of biofunctional PEG and shown to induce neural cell aggregation in culture media. ° ... 

Schmedlen RH, Masters KS. West JL 2002) Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering. Biomaterials 23 4325-4332... 

Mann, B. K., Tsai, A. T., Scott-Burden, T., West, J. L. Modification of surfaces with cell adhesion peptides alters extracellular matrix deposition. Biomaterials. 1999, 20, 2281-2286. 

R.G. LeBaron, K.A. Athanasiou, Extracellular matrix cell adhesion peptides functional applications in orthopedic materials. Tissue Eng. 6 (2000) 85-103. 

In 2D cell culture on chip, cell adhesion has been extensively studied on patterned surfaces for it is critical to cellular functions. Micropattems have been used to study the cellular interactions with various materials such as metals, polymers, self-assembled monolayers, extracellular matrix proteins, cell adhesion peptides, and other bioactive molecules. The physical and chemical properties of a substrate affect the attachment and growth of cells on it, and many studies have demonstrated that different topographical features of a surface affect cell attachment. Glass, sflicon, and polydimethylsiloxane (PDMS) are widely used as substrate materials of ceU culture microchips. 

Anticoagulants, e.g., heparin and hirudin, antimicrobials, cell adhesion peptides, cell adhesion proteins, negative surface charge plasma polymerized coating, thrombolytics Tissue Adhesives... 

In one study [110, 111], the cell adhesion peptide ROD was chemically attached to the PVA-C surface using a more simplihed functionalization reaction route than that reported for glutaraldehyde-crosslinked PVA (Fig. 13) and endothelization was demonstrated on the functionalized surface (Fig. 14). It is interesting that the chemical reactions required for RGD bonding are simpler than those used for... 

Recently, Langer and co-workers have attached cell adhesion peptide sequence such as RGD onto co-polymers of lactides containing lysine residues (Barrera, 1993). These materials exhibit improved cell interactions in comparison vdth the homopol) mer of lactic acid. They have also s)Tithesized comb polymers containing poly(L lysine) pendant groups (Hrkach, 1995) by the nucleophilic ring opening polymerization of the N-carboxy anhydride of -Z-L-lysine, which was initiated by the -amino terminus of the lysine residue in the co-polymer backbone. 

Ito Y, Kajihara M, Imanishi Y. Materials for enhancing cell adhesion by immobilization of cell-adhesive peptide. J Biomed Mater Res 1991 25 1325-37. 

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