Glutaraldehyde crosslinking

A glutaraldehyde crosslinking analysis of C12E8 solubilized H,K-ATPase revealed that only particles of M, = 175 kDa exhibited K -ATPase activity [70]. This size suggests that the functional H,K-ATPase unit is a heterodimer of the catalytic a subunit and the P subunit, since the molecular mass of 175 kDa is probably too small to be a homodimer of the catalytic subunit. 

In the current study we report the investigation of the thermal denaturation behavior of glutaraldehyde modified -D-glucosidase by differential scanning microcalorimetry (DSC), along with the effect of glutaraldehyde crosslinking on the pH/activity-stability of the enzyme at elevated temperatures. 

UOP Ketomax 100 S. olivochromogenes PEI-treated ceramic alumina with glutaraldehyde crosslinked, purified G.l.Rohrbach, 1981. 5.2... 

Figure 12. Release of glucose oxidase from a glutaraldehyde crosslinked partially deacetylated chitin. External lysozyme concentration 2 mg/mL. Weight of gel 18 mg. Amount of glucose oxidase in gel 3 mg 1.5 mg 0.3 mg.

Figure 3. Method II glutaraldehyde crosslinked collagen film removed after 90 days subcutaneous implantation in rabbits. X128...

Figure 4, Method 11 glutaraldehyde crosslinked composite collagen fiber-soluble collagen film removed after 90 days intramuscular implantation in...

Trametes versicolor laccase Glutaraldehyde crosslinking to pH = 6.0, aeration of cell 0.45 0.06 [130] ... 

The fluoride formed was indicated at a fluoride sensitive electrode. The substrate, 4-fluorophenol, has a high reaction rate and a favorable diffusion behavior. The authors observed no interferences by ascorbic acid or uric acid glutathione interfered above 0.87 mg/ml. When immobilized by glutaraldehyde crosslinking with BSA, the enzymes were stable for 30 days. 

For glucose determination the part of the Teflon membrane covering the Au cathode was covered by a cellulose membrane containing GOD immobilized by glutaraldehyde crosslinking. The response time for glucose was 5-10 min and a linear concentration dependence was obtained between 0.1 and 1 mmol/1. 

Posadaka and Macholan (1979) and Matsumoto et al. (1981) combined glutaraldehyde-crosslinked ascorbate oxidase with O2 electrodes. The obtained sensors were applied to the determination of vitamin C in food samples. The only compounds interfering were L-iso-ascorbic acid and triosereductone. 

Glutaraldehyde crosslinks proteins and may alter protein configuration and mask epitopes. Because of its cross-linking properties, penetration of reagents may be impaired. For immunocytochemistry, it is usually used in low concentration in combination with formaldehyde. 

Fig. 14 SEM images showing the microstmcture of (a) glutaraldehyde-crosslinked scaffold and (b) the bilayer dermal equivalent [182]...

In the above sequence, the PEI is chemi-adsorbed onto the surface of the silica particles in the PVC matrix and confers organic functionality to it. The glutaraldehyde crosslinks the PEI rendering it totally insoluble and also acts as a leash for the enzyme pendent group with which it is subsequently reacted. 

Ruiz M., Sastre A., Zikan M.C., Guibal E. Palladium sorption on glutaraldehyde-crosslinked chitosan in fixed-bed systems. Journal of Applied Polymer Science 2001 81 153-165. 

Ldpez-Gallego F, Betancor L, Hidalgo A et al. (2005) Preparation of a robust biocatalyst of D-amino acid oxidase on Sepabeads supports using the glutaraldehyde crosslinking method. Enzyme Microb Technol 37 750-756... 

Xiong, W. W., Wang, W, R, Zhao, L., Song, Q., and Yuan, L. M. Chiral separation of (R,5 )-2-phenyl-l-propanol through glutaraldehyde-crosslinked chitosan membranes. J. Membr. Sci. (2009) 328,268-272. 

Maji, T. K., Barmah, J., Dube, S., and Hussain, M. R. (2007). Microencapsulation of Zanthoxylum limonella oil (ZLO) in glutaraldehyde crosslinked gelatine from mosquito repellent application. Bioresource Technology, 98, 840-844. 

Alonso, N., Lopez-CaUego, F., Betancor, L., Hidalgo, A., Mateo, C., Guisan, J.M., et al., 2005. Immobilization and stabilization of glutaryl acylase on aminated sepabeads supports by the glutaraldehyde crosslinking method. J. Mol. Catal. B Enzym. 35, 57—61. 

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