Peptid-bound

The T cell receptor is a clonotypic, T cell specific surface receptor that mostly recognizes peptides bound to MHC molecules on antigen-presenting cells. 

Boateng S, Lateef SS, Crot C, Motlagh D, Desai T, Samarel AM, Russell B, and Hanley L. Peptides bound to silicone membranes and 3D microfabrication for cardiac cell culture. Adv Mater, 2002, 14, 461 63. 

W. Transferred cross-correlated relaxation complements transferred NOE structure of an IL-4R-derived peptide bound to STAT-6. J. Am. Chem. Soc. 1999, 121, 1949-1953. 

It has recently been claimed that peptide-bound N-hydroxy amino acids occur in human brain tumor, in virus-induced tumor of mouse spleen, and in carcinogen-induced tumors in the rat (112). 

A similar approach has been described by the same authors for the synthesis of related cyclic peptidomimetics [44]. A set often nucleophiles was employed for the substitution of the chlorine atom of the cyclic triazinyl-peptide bound to the cellulose membrane. By virtue of the aforementioned rate enhancement effects for nucleophilic substitution of the solid-supported monochlorotriazines, these reactions could be rapidly carried out by microwave heating. All products were obtained in high purity, enabling systematic modification of the molecular properties of the cyclic peptidomimetics. 

According to Stein (S8), 1761-2459 mg of amino adds appears in 24 hours urine collection from normal subjects after its acid hydrolysis. The estimations performed by Muting (M4) showed that the average content of amino acids in 24-hour specimens of urine derived from 20 normal males and the same number of normal female subjects was 2221 and 2288 mg, respectively. The results of these two authors do not, however, represent exclusively the amount of peptide-bound amino acids, but rather the whole of these compounds liberated as a result of the acid... 

Peptide-bound Amino Acids in 24-Hour Specimens of Urine Derived from 40 Normal Subjects ... 

Peptide-bound amino acids 20 Males 20 Females ... 

FIGURE 5 (A) Schematic of the revised peptidomimetic analysis of FK506. (B) Model of a peptide bound to FKBP12. 

Morpholine (14) is used as base for detaching protected peptides bound to the 9-hydroxyl group of Fm-NHC02-resin, but it is not compatible with 0-glycosylatcd serines. 

Fig. 6. (a) (b) Replacement of a peptide-bound l-hydroxy-2-pyridinone unit by a catechol unit on a tris(ammoethyl)aniine (tren) cap. (c) A tren-capped hexadentate ligand with two 3-hydroxy-2-pyridinone (3,2-hopo) and one terephthalamide (tarn) chelating units. 

Fig. 6 HSQC experiment of the NBD peptide bound to NEMO for the measurement of HNN-HNHof dipole-dipole CCR. The resonance assignment is indicated...

Using 18 trNOE-derived distance restraints and 13 trCCR-derived backbone torsion angle restraints, structure calculations using distance geometry and simulated annealing [48] resulted in a well-defined structure of the IKK/1-derived peptide bound to NEMO. The backbone structure is displayed in Fig. 7B and is compared with the result of the calculation carried out using the trNOE-derived distance restraints alone. It is obvious from Eig. 7 that only the combination of the trNOE- and trCCR-derived restraints results in the structure elucidation of the bound conformation of this peptide. 

Fig. 7 Solution of structure calculations of the NBD-peptide bound to NEMO. A Only trNOE-derived distance restraints were used in the calculation. The structure is not defined by the distance restraints alone. B trNOE-derived distance restraints and trCCR-derived torsion angle restraints were combined. These restraints complement each other and are sufficient to define the NEMO-bound conformation of the IKK/8-derived peptide. In both cases ten solutions were superimposed...

Figure 8. Antigenic sites in the fusion region of influenza virus hemagglutinin of strains A and B. The sites have been localized and their antigenic activity confirmed by synthesis and reactivity with rabbit, goat, and mouse antivirus and antihemagglutinin antibodies. Also, each of the peptides bound considerable amounts of anti-influenza antibodies from sera of human individuals after influenza attack. Reproduced with permission from Ref. 29.

NMR spectroscopy has been proposed as a versatile tool for the analysis of complex mixtures, combinatorial libraries, and heterogeneous samples, such as peptides bound to resins during solid-phase synthesis. 

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