Cationic Antimicrobial Peptide Derived from Polyhydroxyalkanoates

5 Cationic Antimicrobial Peptide Derived from Polyhydroxyalkanoates 

The modification of DPI8 through the conjugation of (R)-3-hydroxydecanoic acid unfortunately resulted in greater cytotoxicity towards both cancer and normal cells. However, the new peptide derivative was still 3 x more toxic to cancer cells compared with human umbilical vein endothelial cells. 

Furthermore, the induction of apoptosis in HeLa (a cell type in an immortal cell line used in scientific research it is the oldest and most commonly used human cell line and was derived from cervical cancer cells from Henrietta Lacks, a patient who eventually died of cancer) and MiaPaCa cells (human pancreatic carcinoma cells) was evident within 2 h, with the progression of cells through early and into late apoptosis. The levels of apoptosis occurring suggested that this was the dominant mode of cell death and was evident by the levels of late apoptosis/necrosis at 24 h. Mitochondria are involved in both apoptotic and necrotic death processes, influenced by the level of adenosine triphosphate and the activation of caspases [78]. 

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