Carrots fractions

Distributions prepared from single lot of diced dehydrated carrots by grinding through food chopper and separating ground material into fractions by sieves. 

Chromatography may be necessary to separate some carotenoids. A saponified carotenoid extract from carrots was applied to OCC on MgO and two fractions were eluted. Successive crystalhzation with petroleum ether and MeOH was carried out to obtain a- and P-carotene crystals, with 99 and 98% purity levels, respectively. ... 

Comparison of the MHR with non-modified pectic hairy regions of apple cell wall, isolated in a mild and defined way, revealed great resemblance indicating that the modifications of the MHR during enzymic liquefaction were only minor. Analogous MHR fractions could be isolated from potato fibre, pear, carrot, leek, and onion tissue. 

The structural features of ceU wall polysaccharides of carrots have been studied by Stevens and Selvendran (1984) and Massiot et al.(1988). Plat et al.(1991), Ben Shalom et al.(1992) and Massiot et al.(1992) investigated the changes in pectic substances of carrots after blanching, dehydration and extended heat treatment. Data on the changes in ceU waU polysaccharides of canned carrots are lacking. This study aims to investigate the effect of preheating time at low temperature and the addition of CaCL on texture and on the composition of various pectin fractions of carrots canned by conventional and by a new process. 

The AIS of fresh and canned carrots were sequentially fractionated into water-soluble pectin (WSP), oxalate-soluble pectin (OXSP), acid-soluble pectin (HSP), alkah-soluble pectin (OHSP), hemicellulose and cellulose. The distribution of uronic acid among various fractions is presented in Table 1. WSP of fresh carrots accounted for 19.0% of the total pectin, while OXSP constituted 29.6%. HSP represented the lowest (12.0%) pectin fraction, whereas OHSP was the highest (35.6%) In the hemicellulose and cellulose fractions significant amounts of uronic acid were found. Heat treatments during canning altered the proportion... 

Figure 5. HPSEC elution pattern of pectin fractions of canned carrots preheated at 65°C for different times, (a) WSP, (b) OXSP, (c) HSP,...

A comparison of the ceU wall fractions of carrots canned by both processes is given in Table 4. Heat treatment during canning process resulted in the solubilization of the major part of HSP and OHSP. This was reflected on an increase in WSP content in carrots canned by conventional process, in OXP content in carrots canned by conventional process with CaCh addition and in WSP and OXP in carrots canned by the new process. The total uronoides content recovered from conventionally canned carrots was 21% lower as compared with... 

Effect of CaCk on the distribution of uronic acid in the fractions of alcohol insoluble solids of carrots canned by conventional and by a new process (as mg AUA/100 g fresh weight)... 

DM and DAc of various pectin fractions except those of OHSP (due to deesterification during extraction) are summarized in Table 5. The DM of WSP of conventionally canned carrots did not significantly differ from that of fresh carrots,while in the presence of CaCk a considerable increase was observed. This increment can be ascribed to cross-Unking of water soluble-low methylated... 

Degree of methylation (DM%) and degree of acetylation (DAc%) of the pectin fractions of carrots canned by conventional and by a new process in the presence of CaCk Fraction Fresh Conv. Conv.+ New +... 

The third described enzyme form with pH optimum about 4.7 [11, 4], we found in Fraction C - the fraction from carrot roots pulp (Fig. 2). We supposed that this form of exopolygalacturonase is relatively strongly bound on carrot cell walls and so it can be released only by higher salt concentrations. The approximative molecular mass determination on Superose 12 (Fig. 3c) showed the molecular mass about 50 000 for this form and the second, with more acidic pH optimum, form present in the fraction. The further characterization of these enzymes showed the exopolygalacturonase with pH optimum 4.7 to be identical with enzyme described sooner by Pressey and Avants [4] and exopolygalacturonase with pH optimum 3.8 to be identical with the enzyme from Fraction A. In conclusion, the exopolygalacturonase form with pH optimum 3.8 can be considered to be the main enzyme form present in carrot roots. 

Pendlington aw, Meuree-Vanlaethem N, Brookes A (i<)g6) The Method Specific Certification of the Mass Fraction of Dietary Fibre in Lyophilised Haricot Beans, Carrot, Apple, Full Fat Soya Flour and Bran Breakfast Cereal Reference Materials CRMs 514, 515, 516, 317 and 518. European Commission Report EUR 17451 EN, Luxembourg. 

Catalase inhibition. Water extract of the fresh root, administered intragastrically to infant mice at a dose of 50 mL/kg, was active. The treatment was administered for seven successive days, followed by a single dose of 20% v/v CCI4 in olive oil subcutaneously at 1 mL/kg on the last day 1 hour after the administration of the carrot extract " . Chloroform-methanol (9 1) fraction of the ethanol (95%) extract, ethyl acetate fraction of the water extract, and chloroform-soluble fraction of the water extract of the seed were active on the nonpregnant rat uterus° L... 

Majumder, P. K., S. Dasgupta, R. K. Mukhopadhaya, U. K. Mazumdar, and M. Gupta. Anti-steroidogenic activity of the petroleum ether extract and fraction 5 (fatty acids) of carrot (Daucus carota L.) seeds in mouse ovary. J Ethnopharmacol 1997 57(3) 209-212. 

Isolation of Plant Cell Walls and Fractionation of Cell Wall Polysaccharides Materials Plant material, fresh and free from bruises and pathogens Ethanol Carrot (for leafy materials optional) Toluidine blue 0 (see recipe) Phloroglucinol-HCl solution (see recipe) Nail varnish or other sealant Iodine in potassium iodide solution (see recipe) Scalpel Double-edged razor blades Watch glass Artists brush, small Compound light microscope with appropriate glass microscope slides and coverslips... 

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