Capillary transfer characteristics

The present model takes into account how capillary, friction and gravity forces affect the flow development. The parameters which influence the flow mechanism are evaluated. In the frame of the quasi-one-dimensional model the theoretical description of the phenomena is based on the assumption of uniform parameter distribution over the cross-section of the liquid and vapor flows. With this approximation, the mass, thermal and momentum equations for the average parameters are used. These equations allow one to determine the velocity, pressure and temperature distributions along the capillary axis, the shape of the interface surface for various geometrical and regime parameters, as well as the influence of physical properties of the liquid and vapor, micro-channel size, initial temperature of the cooling liquid, wall heat flux and gravity on the flow and heat transfer characteristics. 

Weislogel MM, Lichter S (1998) Capillary flow in an interior corner. 1 Eluid Mech 373 349-378 Wu PY, Little WA (1984) Measurement of the heat transfer characteristics of gas flow a fine channels heat exchangers used for microminiature refrigerators. Cryogenics 24 415 20 Xu X, Carey VP (1990) Film evaporation from a micro-grooved surface an approximate heat transfer model and its comparison with experimental data. J Thermophys 4(4) 512-520 Yarin LP, Ekelchik LA, Hetsroni G (2002) Two-phase laminar flow in a heated micro-channels. Int J Multiphase Flow 28 1589-1616... 

The smaller the internal diameter of a capillary column the more efficient the column is for a given stationary phase film thickness on the capillary wall. This is because the mass transfer characteristics of the column are improved with the analyte being able to diffuse in and out of the mobile phase more frequently because of the shorter distance for transverse diffusion (Ch. 10 p. 201). 

Despite the improved mass transfer characteristics of the "plug-like" flow profiles observed in MECC, "intra-column" resistance to mass transfer is significant at higher flow velocities (i.e., at high applied voltages). Although not as dramatic as in our work with hydrostatically-pumped open capillary LC, we have observed improvements in efficiency with the MECC technique when column diameter is reduced. This is illustrated in Figure 6. 

It absorbs a great fraction of moisture from the highly diluted suspension of biomaterials subject to drying. This alters the heat and mass transfer characteristics as the moisture is to be evaporated from a capillary-porous solid instead of from a liquid spray. 

Calame JP, Myers RE, Binari SC, Wood FN, Garven M (2007) Experimental investigation of micro-channel coolers for the high heat flux thermal management of GaN-on-SiC semiconductor devices. Int J Heat Mass Transfer 50 4767-4779 Celata GP, Cumo M, Zummo G (2004) Thermal-hydraulic characteristics of single- phase flow in capillary pipes. Exp Thermal Fluid Sci 28 87-95 Celata GP (2004). Heat transfer and fluid flow in micro-channels. Begell House, N.Y. 

Two-phase flow characteristics of capillaries are known to be significantly different from the characteristics of larger channels, and consequently the existing vast literature associated with the phenomenology of change-of-phase heat transfer and two-phase flow hydrodynamic processes generally do not apply to capillaries. 

For a while now, the problem of flow and heat transfer in heated capillaries has attracted attention from a number of research groups, with several applications to engineering. The knowledge of the thermohydrodynamic characteristics of capillary flow with evaporative meniscus allows one to elucidate the mechanism of heat and mass transfer in porous media, to evaluate the efficiency of cooling system of electronic devices with high power density, as well as to optimize MEMS. 

Two-phase flows in micro-channels with an evaporating meniscus, which separates the liquid and vapor regions, have been considered by Khrustalev and Faghri (1996) and Peles et al. (1998, 2000). In the latter a quasi-one-dimensional model was used to analyze the thermohydrodynamic characteristics of the flow in a heated capillary, with a distinct interface. This model takes into account the multi-stage character of the process, as well as the effect of capillary, friction and gravity forces on the flow development. The theoretical and experimental studies of the steady forced flow in a micro-channel with evaporating meniscus were carried out by Peles et al. (2001). These studies revealed the effect of a number of dimensionless parameters such as the Peclet and Jacob numbers, dimensionless heat transfer flux, etc., on the velocity, temperature and pressure distributions in the liquid and vapor regions. The structure of flow in heated micro-channels is determined by a number of factors the physical properties of fluid, its velocity, heat flux on... 

The quasi-one-dimensional model used in the previous sections for analysis of various characteristics of fiow in a heated capillary assumes a uniform distribution of the hydrodynamical and thermal parameters in the cross-section of micro-channel. In the frame of this model, the general characteristics of the fiow with a distinct interface, such as position of the meniscus, rate evaporation and mean velocities of the liquid and its vapor, etc., can be determined for given drag and intensity of heat transfer between working fluid and wall, as well as vapor and wall. In accordance with that, the governing system of equations has to include not only the mass, momentum and energy equations but also some additional correlations that determine... 

The multiplicity of phenomena characteristic of flow in heated micro-channels determined the content of the book. We consider a number of fundamental problems related to drag and heat transfer in flow of a pure liquid and a two-phase mixture in micro-channels, coolant boiling in restricted space, bubble dynamics, etc. Also considered are capillary flows with distinct interfaces developing under interaction of inertia, pressure, gravity, viscous and capillary forces. 

Principles and Characteristics Thermospray ionisation (TSP) involves introduction of a relatively high flow (0.2-2mLmin ) of solvent into the ion source of a mass spectrometer, and is therefore suitable as an interface for HPLC-MS, using standard bore columns. A vaporiser probe (essentially a resistively heated capillary tube of about 100 xm i.d.) acts as a transfer line for taking solvent and solute into the source. The source is heated to prevent condensation of the solvent, and the temperature of the capillary is chosen so as to ensure vaporisation of the solvent. In this way, a vapour jet is generated, which contains small, electrically charged droplets if the solvent is at least partially aqueous and... 

Principles and Characteristics Although early published methods using SPE for sample preparation avoided use of GC because of the reported lack of cleanliness of the extraction device, SPE-GC is now a mature technique. Off-line SPE-GC is well documented [62,63] but less attractive, mainly in terms of analyte detectability (only an aliquot of the extract is injected into the chromatograph), precision, miniaturisation and automation, and solvent consumption. The interface of SPE with GC consists of a transfer capillary introduced into a retention gap via an on-column injector. Automated SPE may be interfaced to GC-MS using a PTV injector for large-volume injection [64]. LVI actually is the basic and critical step in any SPE-to-GC transfer of analytes. Suitable solvents for LVI-GC include pentane, hexane, methyl- and ethylacetate, and diethyl or methyl-f-butyl ether. Large-volume PTV permits injection of some 100 iL of sample extract, a 100-fold increase compared to conventional GC injection. Consequently, detection limits can be improved by a factor of 100, without... 

The early developments of on-line LC-GC have been reviewed by Davies et al. [496] and Koenigbauer and Major [497]. The selectivity characteristics of the mobile and stationary phases can be optimized to give both a cleaned-up sample and group separation by heart-cutting the desired fraction prior to GC analysis. The LC is usually interfaced to the GC by an uncoated, deactivated GC capillary precolumn to transfer the heart-cut from the LC. This heart-cut from the LC is vaporized to focus the solute at the head of the GC column [498]. The volume of the GC precolumn, the volume of the heart-cut, the GC oven temperature, and carrier gas flow for the concurrent solvent evaporation are carefully matched [499,500]. 

This chapter deals with the validation of capillary electrophoresis (CE) methods. It describes the various validation characteristics, namely accuracy, precision, specificity, detection limit, quantitation limit, linearity, and range in accordance with the official guidelines. Practical aspects related to the calculation of these parameters and factors affecting them in CE analysis have also been described. Validation requirements have been described according to the goal of the method. The chapter contains numerous tables and diagrams to illustrate these ideas. It also covers other related aspects such as instrument qualification, revalidation, and method transfer. 

This chapter sheds light on the different validation requirements and methods to investigate them. Evaluation of the typical validation characteristics, namely accuracy, precision, specificity, DL, QL, linearity, and range in CE, has been discussed in details. Validation in CE is similar to validation in other separation techniques such as HPEC, but in CE, the capillary surface properties and namely the EOF have to be especially addressed. Eurther, the instrument performance has to be carefully considered during validation and method transfer. Here, the condition of the lamp and the thermostating system is of particular importance. 

In addition to providing highly selective separations, there are a multitude of other desired characteristics that a gas chromatographic stationary phase should possess. These properties include high viscosity, low surface tension allowing for wetting of the fused silica capillary wall, high thermal stability, and low vapor pressure at elevated temperatures. The stationary phase solvent should also not exhibit unusual mass transfer behavior. 

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