Capillary electrophoresis capillaries

Capillary Electrophoresis. Capillary electrophoresis (ce) or capillary 2one electrophoresis (c2e), a relatively recent addition to the arsenal of analytical techniques (20,21), has also been demonstrated as a powerful chiral separation method. Its high resolution capabiUty and lower sample loading relative to hplc makes it ideal for the separation of minute amounts of components in complex biological mixtures (22,23). 

Capillary Electrophoresis. Capillary electrophoresis (ce) is an analytical technique that can achieve rapid high resolution separation of water-soluble components present in small sample volumes. The separations are generally based on the principle of electrically driven ions in solution. Selectivity can be varied by the alteration of pH, ionic strength, electrolyte composition, or by incorporation of additives. Typical examples of additives include organic solvents, surfactants (qv), and complexation agents (see Chelating agents). 

Capillary Electrophoresis. Capillaries were first appHed as a support medium for electrophoresis in the early 1980s (44,45). The glass capillaries used are typically 20 to 200 p.m in diameter (46), may be filled with buffer or gel, and are frequendy coated on the inside. Capillaries are used because of the high surface-to-volume ratio which allows high voltages without heating effects. The only limitations associated with capillaries are limits of detection and clearance of sample components. 

Factors Affecting Ionic Migration. Effect of Temperature. pH and Ionic Strength. Electro-osmosis. Supporting Medium. Detection of Separated Components. Applications of Traditional Zone Electrophoresis. High-performance Capillary Electrophoresis. Capillary Electrochromatography. Applications of Capillary El ectrochromatography. ... 

Jimidar, M., Van Ael, W, Shah, R., Redlich, D., and De Smet, M. (2003). Fast method development and rapid analysis using a screening approach for enantiomeric separations in capillary electrophoresis./. Capillary Electrophor. Microchip Technol. 8, 101 — 110. 

Sunday, B. R., Sydor, W., Guariglia, L. M., Obara, J., and Mengisen, R. (2003). Process and product monitoring of recombinant DNA-derived biopharmaceuticals with high-performance capillary electrophoresis./. Capillary Electrophor. 8, 87—99. 

Electromigration techniques capillary electrophoresis capillary electrochromatography... 

Bioluminescence detector Charge-coupled device Contactless-conductivity detector Capillary electrophoresis Capillary electrophoresis-Electrochemistry Collision-induced dissociation Chemiluminescence detector Sodium chlorate-nitrobenzene Commercial off-the-shelf (U.S. Army) Cold Regions Research and Development Center Croatian Mine Action Center Council of Scientific and Industrial Research,... 

Capillary Electrophoresis. Capillary electrophoresis (ce) or capillary zone electrophoresis (czc). a relatively recent addition to the arsenal of... 

Schwartz HE, Ulfelder KJ, Chen F-TA, Pentoney SL Jr (1994) The utility of laser-induced fluorescence detection in applications of capillary electrophoresis. / Capillary Electrophor 1 36-54. 

Capillary electrophoresis Capillary electrophoresis (CE) is used to analyze sodium, potassium, calcium, and magnesium in water samples. The detection is conducted by reverse absorbance measurements. Sufficient separation of the four cations is established with an electrolyte solution of 5 mM imidazole/6.5 mM a-hydroxyisobutyric acid/2 mM 18-crown-6 ether of pEI 4.1 [42]. CE with a contactless conductometric detector is used to determine small anions and cations in water samples from different sources. 2-(N-Morpholino)ethanesulfonic acid/histidine-based (Mes/Elis) electrolytes are used for direct conductivity detection of anions and cations, while ammonium acetate is used for indirect conductivity determination of alkylammonium salts. Eor the simultaneous separation procedure, involving dual-opposite end injection, an electrolyte consisting of 20 mM Mes/EIis, 1.5 mM 18-crown-6 and 20 mM cetyltri-methylammonium bromide provides baseline separation of 13 anions and cations in less than 6 min [43]. Also CE with a capacitively coupled... 

Keywords Capillary electrophoresis Capillary gel electrophoresis Capillary isoelectric focussing Polyelectrolytes Polystyrenesulfonates Polyvinylpyridines Polycarboxybetaines Polyampholytes... 

This chapter treats several separation methods that are not readily classified, including supercritical fluid chromatography, paper chromatography, capillary electrophoresis, capillary electrochromatography, and field-flow fractionation. The use of CE for DNA sequencing is the subject of a feature in the electrophoresis section of this chapter. 

Finally, as a hybrid between pressure-driven chromatography and capillary electrophoresis, Capillary Electrochromatography (CEC) has emerged over the last decade. 

Ptolemy, A.S. and Britz-McKibbin, P. (2006) Sample preconcentration with chemical deri-vatization in capillary electrophoresis capillary as preconcentrator, microreactor and chiral selector for high-throughput metabolite screening. J ChromA, 1106 (1-2), 7-18. 

CE CGE CHOL CIP CPG CTAB CZE dA, dG, dC DBU DEAE DMF DMT DNP DOPE DOTMA EDTA EM EOF ESI-MS Fmoc FPE ICAM-1 Capillary electrophoresis Capillary gel electrophoresis Cholesterol Cahn-Ingold-Prelog nomenclature system for absolute configuration Controlled pore glass Hexadecyltrimethylammonium bromide Capillary zone electrophoresis Deoxyadenosine, deoxyguanosine, deoxycytosine l,8-Diazabicyc o[5.4.0]undec-7-en Diethylaminoethyl- Dimethylformamide Bis(4-methoxyphenyl)phenylmethyl-, (syn. Dimethoxytrityl-) 2,4-DinitrophenyI- Dioleylphosphatidylethanolamine N-[l-(2,3-dioleyloxy)propyl]-N, N, N-trimethylammonium chloride Ethylenediamine tetra-acetic acid Electrophoretic mobility Electro-osmotic flow Electrospray ionization mass spectrometry 9-Fluorenylmethoxycarbonyl-Fluid phase endocytosis Intracellular Adhesion Molecule-1... 

Pacakova V, Coufal P, Stulik K, and Gas B (2003) The importance of capillary electrophoresis, capillary electrochromatography and in chromatography in separations of inorganic ions. Electrophoresis 24 1883-1891. 

Capillary electrophoresis. Capillary electrophoresis provides in general the same information as ion chromatography and is in a sense an alternative technique for this type of application. However, the principle is different because the analyte separation... 

ID Separation of Proteins There has been considerable research devoted to tbe development of nricioflu-idic platforms capable of performing small-scale protein separations. In addition to diffusion based methods [3], nearly every type of electrokineticaUy driven separation has been demonstrated in a cbip-based platform, including free-flow electrophoresis, capillary electrophoresis, capillary gel electrophoresis, isoelectric focusing (lEF), micellar electrokinetic chromatography and capillary electrochemical chromatography. 

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