Capillary affinity

Amini, A., Pettersson, C., and Westerlund, D. (1997). Enantioresolution of disopyramide by capillary affinity electrokinetic chromatography with human alphal-acid glycoprotein (AGP) as chiral selector applying a partial filling technique. Electrophoresis 18, 950—957. 

T Arai, M Ichinose, H Kuroda, N Nimura, T Kinoshita. Chiral separation by capillary affinity zone electrophoresis using an albumin-containing support electrolyte. Anal Biochem 217 7-11, 1994. 

T Arai, N Nimura, T Kinoshita. Investigation of enantioselective ofloxacin-albumin binding and displacement interactions using capillary affinity zone electrophoresis. Biomed Chromatogr 9 68—74, 1995. 

X Wu, RJ Linhardt. Capillary affinity chromatography and affinity capillary electrophoresis of heparin-binding proteins. Electrophoresis 19 2650-2653, 1998. 

Capillary affinity electrophoresis (CAE) or affinity capillary electrophoresis (ACE) — An electrophoretic separation technique (- electrophoresis), in which -> analytes are separated in a capillary, with the -> supporting (background) electrolyte containing substances capable of specific, often biospecific, interactions with the analytes. Ref [i] Riekkola ML, Jonsson jA, Smith RM (2004) Pure Appl Chem 76 443... 

Ljunberg H and Nilsson S. Protein-based capillary affinity gel electrophoresis for chiral separation of 3-adrenergic blockers. 7. Liq. Chromatogr. 1995 18 3685. 

ATIII has been also used as a model protein to test a novel affinity chromatographic system capillary affinity chromatography [9]. Separation quality has been found equivalent to that observed with classical affinity chromatography, whereas the necessary protein amount is strongly reduced to the nanogram level. 

In addition to open-tubular capillary zone electrophoresis, other modes of CE have been used for the separation of proteins and peptides capillary gel electrophoresis, capillary isoelectric focusing, and capillary affinity electrophoresis (406-417). 

Capillary affinity electrophoresis (CAE) based on specific carbohydrafe-protein interaction, is a valuable method in carbohydrate characterization. A variety of carbohydrate-binding proteins (e.g., lectins) specific to certain oligosaccharide structures are available and have been exploited by different research groups to characterize glycoprotein pharmaceuticals [145,146],... 

Nakajima, K., Oda, Y., Kinoshita, M. andKakehi, K. Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates. J Proteome Res, 2, 81, 2003. 

Nakajima, K. et al. Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its apphcation to determination of Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology, 14, 793, 2004. 

Baba, Y. Tsuhako, M. Sawa, T. Akashi, M. Yashima, E. Specific base recognition of oligonucleotides by capillary affinity gel electrophoresis using polyacrylamide-poly(9-vinyladenine) conjugated gel. Anal. Chem. 1992, 64 (17), 1920 1925. 

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