Glycoprotein characterization

It is also becoming more common to combine IEC with other chromatographic methods to optimize glycoprotein characterization. Takahashi160 has developed a three-dimensional sugar mapping technique utilizing three different HPLC columns a DEAE column, a hydrophobic octadecylsilica... 

McGregw JL, Brodiier J, Wild F, Follea G, Trzedak MC, James E, Dechavanne M, McGregor L, Clemetson KJ. Monodonal antibodies against platdet membrane glycoproteins. Characterization and effect on platdet function. Eur JBiocfaem 1983 131 427-36. 

Balaguer, E. and Neusuess, C. (2006) Glycoprotein characterization combining intact protein and glycan analysis by capillary electrophoresis-electrospray ionization-mass spectrometry. Anal Chem, 78 (15), 5384-5393. 

A glycoprotein with affinity for the Fc region of immunoglobulin G has been isolated from extracts of cultured cells infected with Herpes simplex virus type 1. Evidence is provided that it could account for at least some, and possibly all, of the Fc-binding activity detected on the surfaces of infected cells. The glycoprotein accumulates in two electrophoretically distinct forms (mol. wts. 8.0 and 6.5 X lO ) and is distinct from glycoproteins characterized previously from this virus. 

Basak S, Pritchard DG, Bhown AS, Compans RW. (1981) Glycosylation sites of the influenza viral glycoproteins characterization of tryptic glycopeptides from the A/USSR (HlNl) hemagglutinin glycoprotein. J. Virol 37, 549-558. 

The mechanism of inhibition has not been characterized, but it is probably related to the ionophoretic properties of these antibiotics. Monensin has been shown to inhibit the intracellular transport of viral membrane proteins of cells infected with Semliki Forest vims (169). The formation of syncytia, normally observed when T-lymphoblastoid cell line (CEM) cells are cocultivated with human immunodeficiency vims (HlV-l)-infected T-ceU leukemia cell line (MOLT-3) cells, was significantly inhibited in the presence of monensin (170). This observation suggests that the viral glycoproteins in the treated cells were not transported to the cell surface from the Golgi membrane. 

Chemokines are small chemotactic cytokines that act as important messenger molecules between cells of the immune system. Chemokines produce their effects by activating a family of G-protein-coupled receptors. Chemokine receptors are all seven-transmembrane glycoproteins that are structurally related. They may be characterized into those that bind to specific ligands, or those that bind several chemokine ligands. There are also virally encoded (viral) chemokine receptors that represent shared receptors that have been transduced into the viral genome during evolutionary history (Premack and SchaU 1996). 

The Na/K ATPase has been extensively purified and characterized, and consists of a catalytic a subunit of around 95 kDa and a glycoprotein 0 subunit of approximately 45 kDa (Skou, 1992). The functional transporter exists as a dimer with each monomer consisting of an a and /3 subunit. Hiatt aal. (1984) have su ested that the non-catalytic jS subunit may be involved in the cottect insertion of the a subunit into the lipid bilayer and, therefore, it is conceivable that a modification of the 0 subunit structure may be reflected by changes in the catalytic activity of the a subunit. Therefore, in studies involving the manipulation of tissue glutathione levels, alterations of intracellular redox state may have an effect on substrate binding at an extracellular site on this ion-translocating protein. 

Khew-Goodall Y., Grillo M., Getchell M., Danho W., et al. (1991). Vomeromodulin, a putative pheromone transporter cloning, characterization, and cellular-localization of a novel glycoprotein of lateral nasal gland. FASEB J 5, 2976-2982. 

Rohrer, J. S., Cooper, G. A., and Townsend, R. R., Identification, quantification, and characterization of glycopeptides in reversed-phase HPLC separations of glycoprotein proteolytic digests, Anal. Biodiem., 212, 7, 1993. 

Xiang SH, Doka N, Choudhary RK, Sodroski J, Robinson JE. Characterization of CD4-induced epitopes on the HIV type 1 gpl20 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS Res Hum Retroviruses 2002 18(16) 1207—1217. 

Glycoproteins similar to the IDGFs are found in mammals and may constitute a novel class of cytokines, some of which are important in inflammation. The best characterized is the human glycoprotein HC gp-39 (= YKL40 16-23% identical to IDGFs), which accumulates in the synovial fluid of rheumatoid arthritis... 

Buhi WC, Alvarez IM, Choi I, Cleaver BD, Simmen FA 1996 Molecular cloning and characterization of an estrogen-dependent porcine oviductal secretory glycoprotein. Biol Reprod 55 1305-1314... 

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