Affinity capillary electrophoresis overview

In liquid chromatography, affinity purification protocols (4-8) have been known for a long time. Naturally, electrophoresis can be used just as well to observe molecular or noncovalent interactions of DNA oligomers, provided the complex has distinct electrophoretic properties different from those of the free molecules. Therefore, affinity capillary electrophoresis (ACE) can be a powerful tool for studying DNA-drug or DNA-biopolymer interactions. Several reviews discussing these aspects of ACE have been published in recent years (9-19). The crucial aspects of DNA in this field are covered comprehensively in a recent overview article (20). 

See alsa Capillary Electrophoresis Overview. Electrophoresis Overview Principies Isoeiectric Focusing Poiyacryiamide Geis Affinity Techniques Ciinicai Appii-cations Nucieic Acids Proteins. 

See also Capillary Electrophoresis Overview. Electrophoresis Principles Isotachophoresis Isoelectric Focusing Polyacrylamide Gels Two-Dimensional Gels Affinity Techniques Blotting Techniques Clinical Applications. Mass Spectrometry Overview Matrix-Assisted Laser Desorption Ionization Time-of-Flight. 

The aim of this chapter is to cover the theoretical and practical aspects of capillary gel electrophoresis. It also provides an overview of the key application areas of nucleic acid, protein, and complex carbohydrate analysis, affinity-based methodologies, as well as related microseparation methods such as ultra-thin-layer gel electrophoresis and electric field-mediated separations on microchips. It also gives the reader a better understanding of how to utilize this technology, and determine which actual method will provide appropriate technical solutions to problems that may have be perceived as more fundamental. Micropreparative aspects and applications are discussed in Chapter 12. 

---